Reviewed, UniProtKB/Swiss-Prot A7FWA9 (AROE_CLOB1)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	CLB_2416

Organism

Clostridium botulinum (strain ATCC 19397 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

441770 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP(+) = 3-dehydroshikimate + NADPH.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7.
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Shikimate dehydrogenase		PRO_1000021274
Regions
	Nucleotide binding	125 – 129	5	NADP By similarity
Sites
	Active site	65	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A7FWA9-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 5BB7CDDE73F6FCF6	FASTA	258	29,691
	10 20 30 40 50 60 MYTTGLIGKN INYSESPEIH NNYYKKNNIP FFYKIFNLKQ DQIDDFIKNL HKNNIKGFNV 70 80 90 100 110 120 TIPYKETILQ YLNDIVYPAD KIGAVNTVAV QEDKLIGYNT DYIGFIKSLQ YYNIQVKNFK 130 140 150 160 170 180 CLIIGSGGSA KCIYYALKEL NARDICIVSR NPEKARLKFE KKVKILNIKD ENKLDRYDLI 190 200 210 220 230 240 VNCTPIGGPN LKEQKPIELK EIKKNCVVYD LNYTPKRSKL LKEAKENGAF IINGEKMLIF 250 QAYSAIGLWC LNGIKGGR

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000726 Genomic DNA. Translation: ABS35687.1.
RefSeq	YP_001384720.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5395119.
GenomeReviews	Gene locus CLB_2416 in contig CP000726_GR.
KEGG	cba:CLB_2416.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR013708. Shikimate_DHase-bd_N. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view]
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROE_CLOB1

Accession

Primary (citable) accession number: A7FWA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)