SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A7FVY7

- PNP_CLOB1

UniProt

A7FVY7 - PNP_CLOB1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, CLB_2276
Organism
Clostridium botulinum (strain ATCC 19397 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851Magnesium By similarity
Metal bindingi491 – 4911Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCBOT441770:GH1E-2251-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:CLB_2276
OrganismiClostridium botulinum (strain ATCC 19397 / Type A)
Taxonomic identifieri441770 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000006379: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329595Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi441770.CLB_2276.

Structurei

3D structure databases

ProteinModelPortaliA7FVY7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61261KH
Add
BLAST
Domaini622 – 69069S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRRIITHD.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7FVY7-1 [UniParc]FASTAAdd to Basket

« Hide

MIHTLETTVA GRKMKVDFGK TGMLSNAAIF MSYGDTVVMI NANASKEPRE    50
GIDFFPLSVE YEERLYSVGK IPGGFIKREG KPSDKSILHA RSIDRPLRPL 100
FPKGYRNDVQ IVNTVLSVEQ DNLPEILAIN GSSLALCLSS IPFTTPVAAV 150
SVGLVDGEFI INPTVAQREN TILDLTVCAT KERVMMVEAG GQEIDEETMY 200
SAIMFGFEEC KNIVAFQEEA VAKFGKTKNE PVLYKADEEV EKEVKSFAFD 250
MIKEAMYIMD KDERNAQLDK VKEKISEEFS EKYEDKVADI AEVIYKTQKE 300
IVRNMLLNED RRPDGRAFDE VRPISCEVGI LPRTHGTGLF TRGLTQVMTV 350
ATLGALGDVQ ILDGIAEEES KRYMHHYNFP SYSVGEVRPL RGPGRREIGH 400
GALAERALEP LIPSQSEFPY TIRLVSEVLS SNGSTSQASV CGSTLALLDA 450
GVPIKRPAAG IAMGLITSED LEKEKVITDI QGIEDFFGDM DFKVAGTEKG 500
ITSIQFDTKI KGLSNSCVKD ALEGAKKARL HILGKIKECI PEPRKELSKY 550
APRTEIICID PEKIRDVIGA GGKVINKIIA DTNVKIEIKE DGKIFVTSNN 600
EPEGVKKAIS IIEGLTKEVV QGEIYLGKVT KTTNFGAFVE ILPGKEGLVH 650
ISKLDFARVE KVEDVVSVGD EILVKVTDID NQGRINLSRK DAIAKKEEEK 700
DK 702
Length:702
Mass (Da):77,454
Last modified:September 11, 2007 - v1
Checksum:i70A40D92A1091252
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000726 Genomic DNA. Translation: ABS35444.1.
RefSeqiYP_001384587.1. NC_009697.1.

Genome annotation databases

EnsemblBacteriaiABS35444; ABS35444; CLB_2276.
GeneIDi5397699.
KEGGicba:CLB_2276.
PATRICi19359126. VBICloBot110701_2198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000726 Genomic DNA. Translation: ABS35444.1 .
RefSeqi YP_001384587.1. NC_009697.1.

3D structure databases

ProteinModelPortali A7FVY7.
ModBasei Search...

Protein-protein interaction databases

STRINGi 441770.CLB_2276.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS35444 ; ABS35444 ; CLB_2276 .
GeneIDi 5397699.
KEGGi cba:CLB_2276.
PATRICi 19359126. VBICloBot110701_2198.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi RRIITHD.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CBOT441770:GH1E-2251-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19397 / Type A.

Entry informationi

Entry nameiPNP_CLOB1
AccessioniPrimary (citable) accession number: A7FVY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi