Reviewed, UniProtKB/Swiss-Prot A7FVS5 (BIOD_CLOB1)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	CLB_2199

Organism

Clostridium botulinum (strain ATCC 19397 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

441770 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000019556
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7FVS5-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 3CEEEBE362A5C853	FASTA	227	25,363
	10 20 30 40 50 60 MARGIFITAT GTDIGKTYVT ALIIKRLRET NINCGYYKAA LSGAERRDGK LIAGDANYVY 70 80 90 100 110 120 NIANIKGDPN DAVSYIFQQA VSPHLAAKLN NVEISMERIK KDFYSIKNKY DYITVEGSGG 130 140 150 160 170 180 IVCPISTGKE RIMLHNIIKI FKLPAIVVAD AGLGTINSTI LTLQYMKEKN ISVKMILLNN 190 200 210 220 YNHKDIIHIE NKGYLSDNLL IPVYTCNKNA NNLEIPVEKL IEIYEEI

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000726 Genomic DNA. Translation: ABS33109.1.
RefSeq	YP_001384512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7FVS5.
Genome annotation databases
GeneID	5395801.
GenomeReviews	Gene locus CLB_2199 in contig CP000726_GR.
KEGG	cba:CLB_2199.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KGVFITA.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CLOB1

Accession

Primary (citable) accession number: A7FVS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)