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A7FUT5 (NADK_CLOB1) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:CLB_1816
OrganismClostridium botulinum (strain ATCC 19397 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier441770 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281NAD kinase HAMAP-Rule MF_00361
PRO_1000120843

Regions

Nucleotide binding61 – 622NAD By similarity
Nucleotide binding134 – 1352NAD By similarity
Nucleotide binding175 – 1806NAD By similarity

Sites

Active site611Proton acceptor By similarity
Binding site1451NAD By similarity
Binding site1641NAD By similarity
Binding site2341NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A7FUT5 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 0014EA8B8EE1A1A2

FASTA28131,690
        10         20         30         40         50         60 
MKNIGININT DKDISRNILD KIFQYIHEEC SEAKIKVFYD SKGLDNEESR ALDAVMVLGG 

        70         80         90        100        110        120 
DGTILGTARA LAKYDVPIFG INRGHLGFLA EIELEDCKKA IKNLFKGQYK IEDRIMLKCD 

       130        140        150        160        170        180 
LKGIDKKDDF LALNDIVLTK GNLSRIVKYS IYVDDVWYTT FVADGVIVAT PTGSTAYSLS 

       190        200        210        220        230        240 
AGGPIVYPDL DVLEIAPICP HSLGIRPILL NGNSKINIRV LKKYEDPVLT IDGQRYKKVT 

       250        260        270        280 
VNEVTISKSE YKCRLIKFKD KDYFKILRTK ISYRSRECEG E 

« Hide

References

[1]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19397 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000726 Genomic DNA. Translation: ABS32452.1.
RefSeqYP_001384138.1. NC_009697.1.

3D structure databases

ProteinModelPortalA7FUT5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING441770.CLB_1816.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS32452; ABS32452; CLB_1816.
GeneID5396379.
KEGGcba:CLB_1816.
PATRIC19358216. VBICloBot110701_1743.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMARWEALCL.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycCBOT441770:GH1E-1793-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_CLOB1
AccessionPrimary (citable) accession number: A7FUT5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 11, 2007
Last modified: July 9, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families