A7FRV5 (A7FRV5_CLOB1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Probable glycine dehydrogenase [decarboxylating] subunit 1 HAMAP-Rule MF_00712
EC=1.4.4.2 HAMAP-Rule MF_00712

Alternative name(s):
Glycine cleavage system P-protein subunit 1 HAMAP-Rule MF_00712
Glycine decarboxylase subunit 1 HAMAP-Rule MF_00712

Gene names

Name:	gcvPA HAMAP-Rule MF_00712 EMBL ABS32428.1
Ordered Locus Names:	CLB_0737

Organism

Clostridium botulinum (strain ATCC 19397 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

441770 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum A

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00712 SAAS SAAS023010
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP-Rule MF_00712 SAAS SAAS023010
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits By similarity. HAMAP-Rule MF_00712 SAAS SAAS023010
Sequence similarities	Belongs to the GcvP family. N-terminal subunit subfamily. HAMAP-Rule MF_00712

Ontologies

Keywords
Molecular function	Oxidoreductase HAMAP-Rule MF_00712 SAAS SAAS023010 EMBL ABS32428.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A7FRV5 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: BFAD4E1EABA35D0B	FASTA	446	49,543
	10 20 30 40 50 60 MFPYIPNTEK ETKEILDFLS IKSVYELFSD IPDNLKLNRE LDLESSLSEL EVEKRLKALA 70 80 90 100 110 120 LKNKSMEDMT CFLGAGIYDH YIPSVIKHIT GRSEFYTAYT PYQPEVSQGT LQAIFEYQSM 130 140 150 160 170 180 ICALTGMEVS NSSMYDGATA TAEAAILSIV STKRNTIIVS KSVNLDTRKV LKTYLKYRGY 190 200 210 220 230 240 NMVEVDLEDG TTDVDKVINS LDKNVAAVII QSPNFLGLVE DVENMVDAIH KNKSLLIMNV 250 260 270 280 290 300 DPISLGILKS PGELGADIVV GDGQCLGANM SYGGPGFGFM NTTKKLMRKM PGRIVGETED 310 320 330 340 350 360 VEGKRGFVLT LQAREQHIRR EKATSNICSD QTAVAIGAAV YMATLGKEGI KEVAKQCVAK 370 380 390 400 410 420 SHYAYNELIK SGKYKPAFNK PFFKEFVVKG ETSPCEINDK LLENNILGGY DLGKVYSEYE 430 440 NSMLLCVTEK RTREDIDNLV KVMEAI

References

[1]

"Analysis of the Neurotoxin Complex Genes in Clostridium botulinum A1-A4 and B1 Strains: BoNT/A3, /Ba4 and /B1 Clusters Are Located within Plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19397 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000726 Genomic DNA. Translation: ABS32428.1.
RefSeq	YP_001383082.1. NC_009697.1.
3D structure databases
ProteinModelPortal	A7FRV5.
ModBase	Search...
Protein-protein interaction databases
STRING	441770.CLB_0737.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS32428; ABS32428; CLB_0737.
GeneID	5395079.
KEGG	cba:CLB_0737.
PATRIC	19356084. VBICloBot110701_0677.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0403.
HOGENOM	HOG000132025.
KO	K00282.
OMA	MYDGASA.
ProtClustDB	PRK00451.
Enzyme and pathway databases
BioCyc	CBOT441770:GH1E-777-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 1 hit.
HAMAP	MF_00712. GcvPA.
InterPro	IPR020580. GDC-P_N. IPR020581. GDC_P. IPR023010. GDC_P_su1. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11773. PTHR11773. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
PIRSF	PIRSF006815. GcvPA. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A7FRV5_CLOB1

Accession

Primary (citable) accession number: A7FRV5

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)