Reviewed, UniProtKB/Swiss-Prot A7FRV3 (GCST_CLOB1)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	CLB_0735

Organism

Clostridium botulinum (strain ATCC 19397 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

441770 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Aminomethyltransferase HAMAP MF_00259		PRO_1000047657

Sequences

	Sequence		Length	Mass (Da)
	A7FRV3-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: BEE26E513E539D03	FASTA	370	41,629
	10 20 30 40 50 60 MKDLKVTPLR GVYEEYGGKI VDFAGYELPT QFKGFLHEHH TVREKAGLFD VSHMGEAMVT 70 80 90 100 110 120 GKDAGKFIQY LMTNDINVLK DNEVLYTFMC NEDGGVIDDL LVYKFAEDEF FLVINASNKD 130 140 150 160 170 180 KDVKWIMDHK GDFDVEIVDV SDSIAQLAFQ GPLAEEILQK IVDVDLQEIK FFKLKRDVLV 190 200 210 220 230 240 NGKKCLVSRT GYTGEDGFEI YCKPEDAKGL WHAILNAGKE EGAQPIGLGA RDTLRFEASL 250 260 270 280 290 300 LLYGNEMDET ITPLEVGMGF FAKLKIEEDF IGKDALIKQK AEGVTRKLVG FELLDKGIPR 310 320 330 340 350 360 HGYEVIKDGK VIGHVTTGYK SPTLNKAIGL ALVEEQYSKI GTEFNIKVRK KELKAVAIDK 370 RFYTKKTKTK

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000726 Genomic DNA. Translation: ABS34360.1.
RefSeq	YP_001383080.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7FRV3.
Genome annotation databases
GeneID	5397737.
GenomeReviews	Gene locus CLB_0735 in contig CP000726_GR.
KEGG	cba:CLB_0735.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LMTNDIN.
Family and domain databases
HAMAP	MF_00259. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_CLOB1

Accession

Primary (citable) accession number: A7FRV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)