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Protein

Uracil phosphoribosyltransferase

Gene

upp

Organism
Clostridium botulinum (strain ATCC 19397 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.UniRule annotation

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP.UniRule annotation

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase (upp)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 7915-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei104 – 10415-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei194 – 1941Uracil; via amide nitrogenUniRule annotation
Binding sitei200 – 20015-phospho-alpha-D-ribose 1-diphosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT441770:GH1E-171-MONOMER.
UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferaseUniRule annotation (EC:2.4.2.9UniRule annotation)
Alternative name(s):
UMP pyrophosphorylaseUniRule annotation
UPRTaseUniRule annotation
Gene namesi
Name:uppUniRule annotation
Ordered Locus Names:CLB_0181
OrganismiClostridium botulinum (strain ATCC 19397 / Type A)
Taxonomic identifieri441770 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Uracil phosphoribosyltransferasePRO_1000053702Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA7FQG8.
SMRiA7FQG8. Positions 3-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 13995-phospho-alpha-D-ribose 1-diphosphate bindingUniRule annotation
Regioni199 – 2013Uracil bindingUniRule annotation

Sequence similaritiesi

Belongs to the UPRTase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000262754.
KOiK00761.
OMAiVTVIDHP.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.

Sequencei

Sequence statusi: Complete.

A7FQG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVTQIAHP LILHKLALIR DKNTGSKDFR ELVEEVAMLM AYEVTRDLQL
60 70 80 90 100
KEVEIETPIC KTKCKMLSGK KVAIVPILRA GLGMVGGMTS LIPAAKVGHI
110 120 130 140 150
GLYRDEETLK PVEYFCKLPQ DIGDRDVIVT DPMLATGGSA KDAITLLKQK
160 170 180 190 200
GAKHIRLMCL VAAPEGIKEV MDEHPDVDIY VASVDEKLNE KGYVVPGLGD

AGDRLYGTK
Length:209
Mass (Da):22,869
Last modified:September 11, 2007 - v1
Checksum:i8C78BACE20FB358A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000726 Genomic DNA. Translation: ABS32777.1.
RefSeqiWP_003360558.1. NC_009697.1.

Genome annotation databases

EnsemblBacteriaiABS32777; ABS32777; CLB_0181.
KEGGicba:CLB_0181.
PATRICi19354983. VBICloBot110701_0140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000726 Genomic DNA. Translation: ABS32777.1.
RefSeqiWP_003360558.1. NC_009697.1.

3D structure databases

ProteinModelPortaliA7FQG8.
SMRiA7FQG8. Positions 3-209.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS32777; ABS32777; CLB_0181.
KEGGicba:CLB_0181.
PATRICi19354983. VBICloBot110701_0140.

Phylogenomic databases

HOGENOMiHOG000262754.
KOiK00761.
OMAiVTVIDHP.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.
BioCyciCBOT441770:GH1E-171-MONOMER.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B. 1 hit.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUPP_CLOB1
AccessioniPrimary (citable) accession number: A7FQG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.