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A7FPP5

- SYL_CLOB1

UniProt

A7FPP5 - SYL_CLOB1

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium botulinum (strain ATCC 19397 / Type A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei575 – 5751ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCBOT441770:GH1E-215-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:CLB_0226
    OrganismiClostridium botulinum (strain ATCC 19397 / Type A)
    Taxonomic identifieri441770 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000006379: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 813813Leucine--tRNA ligasePRO_1000009325Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi441770.CLB_0226.

    Structurei

    3D structure databases

    ProteinModelPortaliA7FPP5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi40 – 5112"HIGH" regionAdd
    BLAST
    Motifi572 – 5765"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiDKPKYYA.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A7FPP5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNYSTKIDE KWQKKWEENS LYKFNNKNLD KKLYVLEMFS YPSGSKLHAG    50
    HWFNYGPVDS WARFKRMQGY NVFQPMGFDA FGLPAENYAI KTGIHPKDST 100
    FKNIETMETQ LKAMGAMFNW ENEVITCSPD YYKWTQWLFL KLYEKGLAYK 150
    KKAPVNWCPS CNTVLANEQV LDGKCERCDS NVDKKNLEQW FLKITDYADE 200
    LLEKLDELDW PEKTKAMQKH WIGKSVGAEV TFNVADSDLS FNVFTTRVDT 250
    LFGVTYVVLA PENDLVDKLT TPENKAEVES YKTQAKNQSD IERQSITREK 300
    TGVFSGSYAI NPINGKKVPI WIGDYVLNTY GTGCVMAVPA HDERDFAFAT 350
    KYNLPIERVI EGGDSLPYTE YGGMVNSGEF DGLLGNEAKE AVISKLESMN 400
    LGRKKINYRL RDWLVSRQRY WGAPIPIIYC EKCGTVEVPI EQLPVELPYN 450
    VEFSPDGKSP LGKCDDFINT TCPKCGGPAK READTLDTFV CSSWYYLRYP 500
    DNNNEKDAFN PELINKMLPV DKYVGGPEHA CMHLLYARFI TKALRDMGYL 550
    NFDEPFLSLT HQGLILGPDG LKMSKSKGNT ISPDDYIKEF GADVFRMYLM 600
    FGFDYTEGGA WSDDAIKSIG KFVDRVERIL ENAREEIKNS KDNKSTMDKD 650
    EKELNYVRHH SIKSITEDID KMQFNTSIAR LMEFTNALSK YLGIDAIKNA 700
    LFLRESIIDF ITLLAPFAPH FAEEQWKLIG INSSIFNEKW PEFDPKALIK 750
    DEVEIAVQVN GKIRAKINIS TSSSEDEIKE SALNNEDIKN SIGDKEIKKV 800
    IVIKNRLVNI VAK 813
    Length:813
    Mass (Da):92,912
    Last modified:September 11, 2007 - v1
    Checksum:iF4DECBDF3244DC0D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000726 Genomic DNA. Translation: ABS34298.1.
    RefSeqiYP_001382590.1. NC_009697.1.

    Genome annotation databases

    EnsemblBacteriaiABS34298; ABS34298; CLB_0226.
    GeneIDi5396762.
    KEGGicba:CLB_0226.
    PATRICi19355076. VBICloBot110701_0181.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000726 Genomic DNA. Translation: ABS34298.1 .
    RefSeqi YP_001382590.1. NC_009697.1.

    3D structure databases

    ProteinModelPortali A7FPP5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 441770.CLB_0226.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS34298 ; ABS34298 ; CLB_0226 .
    GeneIDi 5396762.
    KEGGi cba:CLB_0226.
    PATRICi 19355076. VBICloBot110701_0181.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi DKPKYYA.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci CBOT441770:GH1E-215-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
      Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
      PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19397 / Type A.

    Entry informationi

    Entry nameiSYL_CLOB1
    AccessioniPrimary (citable) accession number: A7FPP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3