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A7FNS9

- CYSG2_YERP3

UniProt

A7FNS9 - CYSG2_YERP3

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Protein
Siroheme synthase 2
Gene
cysG2, YpsIP31758_3959
Organism
Yersinia pseudotuberculosis serotype O:1b (strain IP 31758)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei248 – 2481Proton acceptor By similarity
Active sitei270 – 2701Proton donor By similarity
Binding sitei306 – 3061S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei382 – 3821S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei411 – 4111S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYPSE349747:GH71-4064-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 2
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 2 (EC:2.1.1.107)
Short name:
Urogen III methylase 2
Alternative name(s):
SUMT 2
Uroporphyrinogen III methylase 2
Short name:
UROM 2
Precorrin-2 dehydrogenase 2 (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase 2 (EC:4.99.1.4)
Gene namesi
Name:cysG2
Ordered Locus Names:YpsIP31758_3959
OrganismiYersinia pseudotuberculosis serotype O:1b (strain IP 31758)
Taxonomic identifieri349747 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000002412: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Siroheme synthase 2UniRule annotation
PRO_0000330581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi349747.YpsIP31758_3959.

Structurei

3D structure databases

ProteinModelPortaliA7FNS9.
SMRiA7FNS9. Positions 1-456.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 204204precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Add
BLAST
Regioni216 – 473258Uroporphyrinogen-III C-methyltransferase By similarity
Add
BLAST
Regioni301 – 3033S-adenosyl-L-methionine binding By similarity
Regioni331 – 3322S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiQASFIMP.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7FNS9-1 [UniParc]FASTAAdd to Basket

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MDYFPIFCQL QHKACLLVGG GEIAERKARL LLDAGALVTV NACEFTPQFH    50
HWADQGQLSL ISGEFVPELL ADKWLVIAAT DQLSVNALVY QSANQQRIFC 100
NVVDDPKRTS FIMPSIIDRS PIMIAVSSGG KAPVLARLLR EKLEALLPQH 150
LGQLAQLAGN LRQRVKQHFA AMTERRRFWE KLLTHDRLAQ SLANNDHVQA 200
DQHVEQLFSA PLTDRGEVVL VGAGPGDAGL LTLKGLQQIQ QADVVVYDRL 250
VSDEVMNLVR RDAERIFVGK QSGHHCVPQE QINQILLQQA QSGKRVVRLK 300
GGDPFIFGRG GEELEELAGY GIPFSVVPGI TAASGCSAYS GIPLTHRDHA 350
QSVRLVTGHA KKEGQLDWAN LAAEKQTLVF YMGLSQAGEI QQQLIQHGMP 400
ATTQVALVEN GTSRHQRVVS GELSQLALLS QQVSSPSLII VGSVVSLREK 450
LNWFSSRHHD DQPKVTECVA HVG 473
Length:473
Mass (Da):51,918
Last modified:September 11, 2007 - v1
Checksum:i3A220E0A0E63E7FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000720 Genomic DNA. Translation: ABS46625.1.
RefSeqiYP_001402907.1. NC_009708.1.

Genome annotation databases

EnsemblBacteriaiABS46625; ABS46625; YpsIP31758_3959.
GeneIDi5384901.
KEGGiypi:YpsIP31758_3959.
PATRICi18637235. VBIYerPse15693_4506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000720 Genomic DNA. Translation: ABS46625.1 .
RefSeqi YP_001402907.1. NC_009708.1.

3D structure databases

ProteinModelPortali A7FNS9.
SMRi A7FNS9. Positions 1-456.
ModBasei Search...

Protein-protein interaction databases

STRINGi 349747.YpsIP31758_3959.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS46625 ; ABS46625 ; YpsIP31758_3959 .
GeneIDi 5384901.
KEGGi ypi:YpsIP31758_3959.
PATRICi 18637235. VBIYerPse15693_4506.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi QASFIMP.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci YPSE349747:GH71-4064-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Yersinia pseudotuberculosis IP31758, the causative agent of Far East scarlet-like fever."
    Eppinger M., Rosovitz M.J., Fricke W.F., Rasko D.A., Kokorina G., Fayolle C., Lindler L.E., Carniel E., Ravel J.
    PLoS Genet. 3:1508-1523(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IP 31758.

Entry informationi

Entry nameiCYSG2_YERP3
AccessioniPrimary (citable) accession number: A7FNS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 11, 2007
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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