A7FM52 (GUAC_YERP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	YpsIP31758_3373

Organism

Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) [Complete proteome] [HAMAP]

Taxonomic identifier

349747 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP-Rule MF_00596
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP-Rule MF_00596
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	GMP reductase HAMAP-Rule MF_00596		PRO_1000061239
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
	Nucleotide binding	216 – 239	24	NADP; ribose moiety By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7FM52 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 4BF8680985AB19DF	FASTA	347	37,492
	10 20 30 40 50 60 MRIEEGLKLG FKDVLIRPKR STLKSRSEVA LERQFTFKHS GWNWSGVPII AANMDTVGTF 70 80 90 100 110 120 RMAEVLASFD ILTAVHKHYT LEQWAEFVKR SPESVLRHVM VSTGTSSADF DKMKQILALS 130 140 150 160 170 180 PSLKFICIDV ANGYSEHFVS FLQRAREACP DKVICAGNVV TGEMVEELIL SGADIVKVGI 190 200 210 220 230 240 GPGSVCTTRV KTGVGYPQLS AVIECADAAH GLGGQIVSDG GCSVPGDVAK AFGGGADFVM 250 260 270 280 290 300 LGGMLAGHDE CEGRVVEENG EKFMLFYGMS SESAMKRHVG GVAQYRAAEG KTVKLPLRGS 310 320 330 340 VDNTVRDIMG GLRSACTYVG ASHLKELTKR TTFIRVAEQE NRVFGTD

References

[1]

"The complete genome sequence of Yersinia pseudotuberculosis IP31758, the causative agent of Far East scarlet-like fever." Eppinger M., Rosovitz M.J., Fricke W.F., Rasko D.A., Kokorina G., Fayolle C., Lindler L.E., Carniel E., Ravel J. PLoS Genet. 3:1508-1523(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP 31758.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000720 Genomic DNA. Translation: ABS46719.1.
RefSeq	YP_001402330.1. NC_009708.1.
3D structure databases
ProteinModelPortal	A7FM52.
SMR	A7FM52. Positions 3-338.
ModBase	Search...
Protein-protein interaction databases
STRING	349747.YpsIP31758_3373.
Proteomic databases
PRIDE	A7FM52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS46719; ABS46719; YpsIP31758_3373.
GeneID	5386649.
KEGG	ypi:YpsIP31758_3373.
PATRIC	18635919. VBIYerPse15693_3868.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
HOGENOM	HOG000165756.
KO	K00364.
OMA	CSCAGDV.
ProtClustDB	PRK05096.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00596. GMP_reduct_type1.
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_YERP3

Accession

Primary (citable) accession number: A7FM52

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program