A7FLY8 (CYSH_YERP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	YpsIP31758_3309

Organism

Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) [Complete proteome] [HAMAP]

Taxonomic identifier

349747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000057434

Sequences

	Sequence		Length	Mass (Da)
	A7FLY8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: D9C60C79D63593DF	FASTA	244	27,852
	10 20 30 40 50 60 MSQFNLSELN ALPKAKQAAA LVLVNGQLEH LTAQERVSWA LDNLPGEFVL SSSFGIQAAV 70 80 90 100 110 120 CLHLVTRQRP DIPVILTDTG YLFPETYRFI DDLTEKLQLN LQVFRAAHSP AWQEARYGKL 130 140 150 160 170 180 WEQGVEGIER YNTLNKVEPM NRALEALGAQ TWFAGLRREQ SGGRSQLPVL ALQRGIFKLL 190 200 210 220 230 240 PIIDWDNRQV YQYLTQHGLS YHPLWEQGYL SVGDTHTTRK WEPGMSEEET RFFGLKRECG LHEG

References

[1]

"The complete genome sequence of Yersinia pseudotuberculosis IP31758, the causative agent of Far East scarlet-like fever." Eppinger M., Rosovitz M.J., Fricke W.F., Rasko D.A., Kokorina G., Fayolle C., Lindler L.E., Carniel E., Ravel J. PLoS Genet. 3:1508-1523(2007) [PubMed: 17784789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP 31758.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000720 Genomic DNA. Translation: ABS47225.1.
RefSeq	YP_001402266.1. NC_009708.1.
3D structure databases
ProteinModelPortal	A7FLY8.
SMR	A7FLY8. Positions 2-244.
ModBase	Search...
Protein-protein interaction databases
STRING	A7FLY8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5384495.
GenomeReviews	Gene locus YpsIP31758_3309 in contig CP000720_GR.
KEGG	ypi:YpsIP31758_3309.
PATRIC	18635779. VBIYerPse15693_3798.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HBG758022.
OMA	TNFRPYE.
ProtClustDB	PRK02090.
Enzyme and pathway databases
BioCyc	YPSE349747:YPSIP31758_3309-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_YERP3

Accession

Primary (citable) accession number: A7FLY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program