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A7FLY4

- CYSG1_YERP3

UniProt

A7FLY4 - CYSG1_YERP3

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Protein

Siroheme synthase 1

Gene
cysG1, YpsIP31758_3305
Organism
Yersinia pseudotuberculosis serotype O:1b (strain IP 31758)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei247 – 2471Proton acceptor By similarity
Active sitei269 – 2691Proton donor By similarity
Binding sitei305 – 3051S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei382 – 3821S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei411 – 4111S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYPSE349747:GH71-3393-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 1
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 1 (EC:2.1.1.107)
Short name:
Urogen III methylase 1
Alternative name(s):
SUMT 1
Uroporphyrinogen III methylase 1
Short name:
UROM 1
Precorrin-2 dehydrogenase 1 (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase 1 (EC:4.99.1.4)
Gene namesi
Name:cysG1
Ordered Locus Names:YpsIP31758_3305
OrganismiYersinia pseudotuberculosis serotype O:1b (strain IP 31758)
Taxonomic identifieri349747 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000002412: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Siroheme synthase 1UniRule annotationPRO_0000330580Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi349747.YpsIP31758_3305.

Structurei

3D structure databases

ProteinModelPortaliA7FLY4.
SMRiA7FLY4. Positions 1-456.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarityAdd
BLAST
Regioni215 – 472258Uroporphyrinogen-III C-methyltransferase By similarityAdd
BLAST
Regioni300 – 3023S-adenosyl-L-methionine binding By similarity
Regioni330 – 3312S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLHQQLAW.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7FLY4-1 [UniParc]FASTAAdd to Basket

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MDYLPLFADL KQRPVLIVGG GEVAARKIEL LHRAGAQVWV VAQTLSSELE    50
QQYQDGRIHW LAQDFLPEQL DNVFLVIAAT NDTVLNAAVF AAADQRCILA 100
NVVDDQPLCS FIFPSIVDRS PLVVAISSSG QAPVLARILR EKLEALLPTR 150
LSDMAAIAGR WRGRVKQHMA SMGERRRFWE HAFSGRFASL ISRGQLTEAE 200
NELQLSLEGQ HRALGEVALV GAGPGDAGLL TLRGLQVMQQ ADVVLYDHLV 250
SPEVLDLVRR DAERICVGKR AGAHSVTQEA TNQLLVTLAQ QGKRVVRLKG 300
GDPFIFGRGG EELQVVAQAG IPFQVVPGVT AAAGATAYAG IPLTHRDHAQ 350
SVTFITGHCR PDGDDLDWQA LARGRQTLAI YMGTVKAAAI SQQLIAHGRS 400
STTPVAVIGR GTRVDQQVLI GTLAQLESLA QQAPTPALLV IGEVVNLHHQ 450
IAWFGQQPQT ESAINPSVVN LA 472
Length:472
Mass (Da):50,901
Last modified:September 11, 2007 - v1
Checksum:iD2D7DB7E4BCF4F98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000720 Genomic DNA. Translation: ABS48251.1.
RefSeqiYP_001402262.1. NC_009708.1.

Genome annotation databases

EnsemblBacteriaiABS48251; ABS48251; YpsIP31758_3305.
GeneIDi5386338.
KEGGiypi:YpsIP31758_3305.
PATRICi18635771. VBIYerPse15693_3794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000720 Genomic DNA. Translation: ABS48251.1 .
RefSeqi YP_001402262.1. NC_009708.1.

3D structure databases

ProteinModelPortali A7FLY4.
SMRi A7FLY4. Positions 1-456.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349747.YpsIP31758_3305.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS48251 ; ABS48251 ; YpsIP31758_3305 .
GeneIDi 5386338.
KEGGi ypi:YpsIP31758_3305.
PATRICi 18635771. VBIYerPse15693_3794.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi LHQQLAW.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci YPSE349747:GH71-3393-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Yersinia pseudotuberculosis IP31758, the causative agent of Far East scarlet-like fever."
    Eppinger M., Rosovitz M.J., Fricke W.F., Rasko D.A., Kokorina G., Fayolle C., Lindler L.E., Carniel E., Ravel J.
    PLoS Genet. 3:1508-1523(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IP 31758.

Entry informationi

Entry nameiCYSG1_YERP3
AccessioniPrimary (citable) accession number: A7FLY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 11, 2007
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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