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A7EV21

- LIPA_SCLS1

UniProt

A7EV21 - LIPA_SCLS1

Protein

Lipoyl synthase, mitochondrial

Gene

SS1G_09179

Organism
Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi130 – 1301Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi135 – 1351Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi161 – 1611Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi168 – 1681Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:SS1G_09179
    OrganismiSclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
    Taxonomic identifieri665079 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeSclerotinia
    ProteomesiUP000001312: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434MitochondrionUniRule annotationAdd
    BLAST
    Chaini35 – 409375Lipoyl synthase, mitochondrialPRO_0000398292Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi665079.A7EV21.

    Structurei

    3D structure databases

    ProteinModelPortaliA7EV21.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OMAiPEEPYNT.
    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A7EV21-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASIVPRFRC LNAPPLRQTS RNILAIPTAT SRSFATEIDV SKPTPIVKRR    50
    PTYFKDKINA VPSFSEFVGL PEKAFTPEDA LELRTAMVGP PGKKRQITRL 100
    PEWLKTPIPD NSNYKKIKKD LRGLNLHTVC EEARCPNISD CWGGSDKSAA 150
    TATIMLMGDT CTRGCRFCSV KTSKAPPPLD PHEPEHTAEA LSRWGLGYVV 200
    LTSVDRDDLA DGGARHFAET IMKIKQKKSS ILVEALTGDY GGDLEMVKLV 250
    AESGLDVYAH NMETTEELTP FVRDRRAKYR QSLKVLEAAK KAKPSLITKT 300
    SIMLGLGETE EALWQALRDL REVDVDVVTF GQYMRPTKRH MKVEEYVTPE 350
    VFEIWRQRAL DLGFLYCASG PLVRSSYKAG EAFIENVLKK RKGVTILSDE 400
    ATGMDKAIV 409
    Length:409
    Mass (Da):45,637
    Last modified:September 11, 2007 - v1
    Checksum:i8EA97891D4BC7425
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476633 Genomic DNA. Translation: EDN93313.1.
    RefSeqiXP_001589458.1. XM_001589408.1.

    Genome annotation databases

    EnsemblFungiiEDN93313; EDN93313; SS1G_09179.
    GeneIDi5485857.
    KEGGissl:SS1G_09179.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476633 Genomic DNA. Translation: EDN93313.1 .
    RefSeqi XP_001589458.1. XM_001589408.1.

    3D structure databases

    ProteinModelPortali A7EV21.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 665079.A7EV21.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EDN93313 ; EDN93313 ; SS1G_09179 .
    GeneIDi 5485857.
    KEGGi ssl:SS1G_09179.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OMAi PEEPYNT.
    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea."
      Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E.
      , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.
      PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 18683 / 1980 / Ss-1.

    Entry informationi

    Entry nameiLIPA_SCLS1
    AccessioniPrimary (citable) accession number: A7EV21
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3