A7ERM5 (ATG26_SCLS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol 3-beta-glucosyltransferase
EC=2.4.1.173

Alternative name(s):
Autophagy-related protein 26

Gene names

Name:	atg26
ORF Names:	SS1G_07979

Organism

Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)

Taxonomic identifier

665079 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Helotiales › Sclerotiniaceae › Sclerotinia

Protein attributes

Sequence length	1435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1435	1435	Sterol 3-beta-glucosyltransferase		PRO_0000318048
Regions
	Domain	211 – 258	48	GRAM 1
	Domain	262 – 360	99	PH
	Domain	760 – 826	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	A7ERM5 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: B2BF72431A06DF92	FASTA	1,435	160,945
	10 20 30 40 50 60 MAPDDESKKR ATRKSTKRWK EEHQVATEIP ERFREGDDED EDCTAGHALN KSVFGMIAAA 70 80 90 100 110 120 SSIVDFNARF DAQSSDEEYD LGKPTRQSSE SHINRSSIDQ KMGKFEGHRR LLSESKILRS 130 140 150 160 170 180 FSRFSSRSKS KSSNTIARGS RTPSPLRESR QEPTSPEIRY SPLQPKDTPV MSRMLEARAE 190 200 210 220 230 240 LSLRPSFEMP RKSKDPAEVD DERTSPDSLA ERLMEIFKFE TPEDVLEEYP CWLMKSVLLQ 250 260 270 280 290 300 GYMYITTKHI CFYAYLPKKA NEVVKSGYLS KSGRHNPKYN RYWFRLKDDV LSYYTNPSDL 310 320 330 340 350 360 YFPSGNIDLR YGISASIVEK EKGKDSTHFT VETHQRKYNF NADSAPSAKE WVKALQKIIF 370 380 390 400 410 420 RSHNDGDSVK ISLPIENIID IEDSRIIDFA DTCKIRIIDN DETYAIDEYF FSFFTNGKLA 430 440 450 460 470 480 LSVLKILVGD TAAQKIPEEL LSPLSTADDS RGSSKRTSFQ VSRDRKLHGP SVQPNAPALE 490 500 510 520 530 540 ETVRATLSPL LAPGAGSPRA STDMSLSSSD IFRRSLDINK HGRRSIDLNR LTTEHHRSNS 550 560 570 580 590 600 ANSISTRRSL STNRLDQEKR TSKQGSSDSY VHSLEEPGSS EALPSASDET QASASQILRG 610 620 630 640 650 660 SDVFLSPTIQ HSPSVACNRN KDEIQGPKSR SLTSPIKSAD VSPTRTQRPQ HPKHAATTGS 670 680 690 700 710 720 VSDSNVGQAE ISSGPSLQSI VKAGSYPLQR AAGFAGYLNR HSKRMSTLLA SESMGYVEKV 730 740 750 760 770 780 SGMWKGGKKH YEEPHGIPRD NEMPSIGDDD DNRDGATPAD RFRDHFALPP DEKLHATYFG 790 800 810 820 830 840 YLQRVLPLYG KIYISDRSFC FRSLLPGTRT KFILPLKDIE NVDKEKGFRF GYSGLVITIR 850 860 870 880 890 900 GHEEIFFEFN QAENRDDCAI TLLQNVETMQ YMQDSGLLTM YERESAEMAG AEHRALIQAR 910 920 930 940 950 960 RGIRSEHDID MHKAADGGSQ SISFDDPRAS ILNFKPTEPL RITCLTIGSR GDVQPYIALC 970 980 990 1000 1010 1020 KGLMAEGHQT KIATHLEFKE WIESHGIEFA PVDGDPAELM RICVENGMFT VSFLKEASSK 1030 1040 1050 1060 1070 1080 FRGWIDDLLS SSWRACQNSD ILIESPSAMA GIHIAEALRI PYFRAFTMPW TRTRAYPHAF 1090 1100 1110 1120 1130 1140 AVPEHKLGGS YNYVTYLMFD TVFWRAISGQ VNRWRQKELN LQATGLEKMQ PNKVPFLYNF 1150 1160 1170 1180 1190 1200 SPSVVVPPLD YSDWIRVTGY WFLDEGANYT PPKDLTDFIA NARADGKRLV YVGFGSIVVA 1210 1220 1230 1240 1250 1260 DSAVLTKTVV ASVLKADVRC ILSKGWSDRL EKKGANNVEI PLPPEIFQIK SAPHDWLFSQ 1270 1280 1290 1300 1310 1320 VDAAAHHGGA GTTGASLRAG IPTIIRPFFG DQFFFGQRVE DLGVGVLIKK INVSVFSRAL 1330 1340 1350 1360 1370 1380 WEATHSERII TKARVLGEQI RKENGVDTAI QSIYRDMEYA KSLIKCKEGK SDDDTLEDSE 1390 1400 1410 1420 1430 ESWTFIGDDT DPELIKRFYD WESMAHSGTL SDKNMMAWSG SDVASAALSP TRKAA

References

[1]

"Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea." Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E. , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M. PLoS Genet. 7:E1002230-E1002230(2011) [PubMed: 21876677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 18683 / 1980 / Ss-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476630 Genomic DNA. Translation: EDN92117.1.
RefSeq	XP_001591353.1. XM_001591303.1.
3D structure databases
ProteinModelPortal	A7ERM5.
ModBase	Search...
Protein-protein interaction databases
STRING	A7ERM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5487401.
KEGG	ssl:SS1G_07979.
Phylogenomic databases
OrthoDB	EOG48KVK8.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K05841.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_SCLS1

Accession

Primary (citable) accession number: A7ERM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program