Reviewed, UniProtKB/Swiss-Prot A7EKT5 (MCR1_SCLS1)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	mcr1
ORF Names:	SS1G_05932

Organism

Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum) [Complete proteome]

Taxonomic identifier

665079 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Helotiales › Sclerotiniaceae › Sclerotinia

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADH-cytochrome b5 reductase 2		PRO_0000330190
Regions
	Transmembrane	31 – 47	17	Potential
	Domain	94 – 199	106	FAD-binding FR-type
	Nucleotide binding	202 – 237	36	FAD By similarity
	Compositional bias	24 – 29	6	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	A7EKT5-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 802B93D76510FC22	FASTA	345	37,819
	10 20 30 40 50 60 MFARQTLRAA QPFRSQYRRY ATESSSSGSN TALYTGVAAA VAGAGYYFMS QGDNAAKVKD 70 80 90 100 110 120 AAKDAEAKAK EALGQGQAKV EGAVGKAAFT GGDQGFISLK LDSVELVNHN TKKFRFELPE 130 140 150 160 170 180 SDQVSGLHVT SALITKYKGP EMQKPAIRPY TPTSDEGEKG FIDLLVKKYP NGVMSEHMHE 190 200 210 220 230 240 MVPGQRLDFK GPIPKYAWSA NKHDHIALIA GGTGITPMYQ LARAIFNNPA DKTKVTLVFA 250 260 270 280 290 300 NVTEEDILLK REFEDLENTY PQRFRAFYVL DNPPKSWKGG KGFINKELLK TVLPEPKSEN 310 320 330 340 IKLFVCGPPG MYKAISGGKV SPSDQGELAG ILKELGYSKD QVYKF

References

[1]

"Annotation of the Sclerotinia sclerotiorum 1980 genome." Birren B.W., Galagan J.E., Lander E.S., Devon K., Nusbaum C., Cuomo C., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Yandava C., Kodira C.D., Zeng Q., Alvarado L., O'Leary S., Dickman M.B., Kohn L., Rollins J. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476627 Genomic DNA. Translation: EDO03451.1.
RefSeq	XP_001593010.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5488857.
KEGG	ssl:SS1G_05932.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_SCLS1

Accession

Primary (citable) accession number: A7EKT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 11, 2007
Last modified:	October 13, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)