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A7EBU5

- BGALB_SCLS1

UniProt

A7EBU5 - BGALB_SCLS1

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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871SubstrateBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Binding sitei133 – 1331Substrate; via amide nitrogenBy similarity
Binding sitei134 – 1341SubstrateBy similarity
Binding sitei192 – 1921SubstrateBy similarity
Active sitei193 – 1931Proton donorSequence Analysis
Binding sitei262 – 2621SubstrateBy similarity
Active sitei305 – 3051NucleophileSequence Analysis
Binding sitei370 – 3701SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:SS1G_02781
OrganismiSclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Taxonomic identifieri665079 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeSclerotinia
ProteomesiUP000001312: Unassembled WGS sequence

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 1008990Probable beta-galactosidase BPRO_0000395233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi268 ↔ 321By similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi594 – 5941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi624 – 6241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi703 – 7031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi782 – 7821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi788 – 7881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi816 – 8161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi826 – 8261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi879 – 8791N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi665079.A7EBU5.

Structurei

3D structure databases

ProteinModelPortaliA7EBU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
InParanoidiA7EBU5.
OMAiYINANTS.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7EBU5-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MLLQSLFAWA LAIGPCIAQN STNSTWPIHN NGLTTQVEWD HYSLMVDGQR
60 70 80 90 100
FFLWSGEFHY WRIPVPELWV DVLQKVKAAG FNTFAIYTHW YFHNPNPNTL
110 120 130 140 150
DFENAAHNFT KIFDLAKELG MFVVFRPGPY VNAESNAGAF PLWLTTGAYG
160 170 180 190 200
ALRNNDERYT EAWTPFWEKV ASIVAPYQFT NGGNVLTYQI ENELGSQWRG
210 220 230 240 250
TPSNKVPNLS SVEYMEALEA SARAHGITIP FQANDPNLNS DSWSKDFYDG
260 270 280 290 300
YGSVDIYGMD SYPACWTCNL TECDSTNGAY KAFNVIDYYD HFEAISPTQP
310 320 330 340 350
SFLPEFQGGS FNPWGGPEGG CPENSPADFA NLFYRNNVGQ RVTAMSLYMI
360 370 380 390 400
YGGTNWGWLA APVVATSYDY SSPISENRMI NDKYAETKLF GHFLRVAKDL
410 420 430 440 450
TKTDRIGTGK TASTNPNVVY SEIRNPDTNA AFYVTIHKES TVGTREEFYI
460 470 480 490 500
NANTSKGAFK IPQKAASIVL NGFQSKIIVT DFNFGSHSLL YSTAEVLSHS
510 520 530 540 550
IVDDQDILAL WMPTGEAGEF VVTGAKSGSV SSCGGCSSVG FYPQGDDLLV
560 570 580 590 600
TISQSKGISV LTFDDGLRVL VMDRSFAYEF WVPVLTADPF SPANETVFVQ
610 620 630 640 650
GPSLVRSAAY SSDGSTLDLT GDNNGTSTQI QVFPPKSVSK VTWNGQVITT
660 670 680 690 700
EKTDYDSLIG SLTGPALDSL TLPTISGWKA NDSLPERLPT YDDSWWVAAD
710 720 730 740 750
HMNTSNPTKP ETLPVLYIDD YGYHVGNHLW RGRFEGSASG VYLSVTGGRA
760 770 780 790 800
FGYSAWLNGE FIGSYLGAAY PDTGKLTLSF SNVTVNSNST NILLVLQDNS
810 820 830 840 850
GHDETSEALN PRGINNATLI SSSTKNFTSW KVTGTAGKPD TAIDPVRGIL
860 870 880 890 900
SEGGLYAERL GWHLPDFDDS EWSSASPSNV SSSAGVTFYR TTVSLAIPTG
910 920 930 940 950
LDVAISFTLK ASPSNAALRV LLFVNGYQYG RFSPWIGNQV EFPVPPGILN
960 970 980 990 1000
YDGDNVIGLS VWRQEEGDES MGVNVGWKVT EAFASSFEPI FDAAYLQPGW

TDERLQYA
Length:1,008
Mass (Da):110,685
Last modified:September 11, 2007 - v1
Checksum:i52E5C90A03793D5E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH476623 Genomic DNA. Translation: EDN99923.1.
RefSeqiXP_001596561.1. XM_001596511.1.

Genome annotation databases

EnsemblFungiiEDN99923; EDN99923; SS1G_02781.
GeneIDi5492844.
KEGGissl:SS1G_02781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH476623 Genomic DNA. Translation: EDN99923.1 .
RefSeqi XP_001596561.1. XM_001596511.1.

3D structure databases

ProteinModelPortali A7EBU5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 665079.A7EBU5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EDN99923 ; EDN99923 ; SS1G_02781 .
GeneIDi 5492844.
KEGGi ssl:SS1G_02781.

Phylogenomic databases

eggNOGi COG1874.
InParanoidi A7EBU5.
OMAi YINANTS.
OrthoDBi EOG7ZGXBD.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea."
    Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E.
    , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.
    PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 18683 / 1980 / Ss-1.

Entry informationi

Entry nameiBGALB_SCLS1
AccessioniPrimary (citable) accession number: A7EBU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3