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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Binding sitei133Substrate; via amide nitrogenBy similarity1
Binding sitei134SubstrateBy similarity1
Binding sitei192SubstrateBy similarity1
Active sitei193Proton donorSequence analysis1
Binding sitei262SubstrateBy similarity1
Active sitei305NucleophileSequence analysis1
Binding sitei370SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:SS1G_02781
OrganismiSclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Taxonomic identifieri665079 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeSclerotinia
Proteomesi
  • UP000001312 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:SS1G_02781.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000039523319 – 1008Probable beta-galactosidase BAdd BLAST990

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi20N-linked (GlcNAc...)Sequence analysis1
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1
Glycosylationi108N-linked (GlcNAc...)Sequence analysis1
Glycosylationi208N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi268 ↔ 321By similarity
Glycosylationi269N-linked (GlcNAc...)Sequence analysis1
Glycosylationi453N-linked (GlcNAc...)Sequence analysis1
Glycosylationi594N-linked (GlcNAc...)Sequence analysis1
Glycosylationi624N-linked (GlcNAc...)Sequence analysis1
Glycosylationi681N-linked (GlcNAc...)Sequence analysis1
Glycosylationi703N-linked (GlcNAc...)Sequence analysis1
Glycosylationi782N-linked (GlcNAc...)Sequence analysis1
Glycosylationi788N-linked (GlcNAc...)Sequence analysis1
Glycosylationi816N-linked (GlcNAc...)Sequence analysis1
Glycosylationi826N-linked (GlcNAc...)Sequence analysis1
Glycosylationi879N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliA7EBU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiA7EBU5.
OMAiYINANTS.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7EBU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLQSLFAWA LAIGPCIAQN STNSTWPIHN NGLTTQVEWD HYSLMVDGQR
60 70 80 90 100
FFLWSGEFHY WRIPVPELWV DVLQKVKAAG FNTFAIYTHW YFHNPNPNTL
110 120 130 140 150
DFENAAHNFT KIFDLAKELG MFVVFRPGPY VNAESNAGAF PLWLTTGAYG
160 170 180 190 200
ALRNNDERYT EAWTPFWEKV ASIVAPYQFT NGGNVLTYQI ENELGSQWRG
210 220 230 240 250
TPSNKVPNLS SVEYMEALEA SARAHGITIP FQANDPNLNS DSWSKDFYDG
260 270 280 290 300
YGSVDIYGMD SYPACWTCNL TECDSTNGAY KAFNVIDYYD HFEAISPTQP
310 320 330 340 350
SFLPEFQGGS FNPWGGPEGG CPENSPADFA NLFYRNNVGQ RVTAMSLYMI
360 370 380 390 400
YGGTNWGWLA APVVATSYDY SSPISENRMI NDKYAETKLF GHFLRVAKDL
410 420 430 440 450
TKTDRIGTGK TASTNPNVVY SEIRNPDTNA AFYVTIHKES TVGTREEFYI
460 470 480 490 500
NANTSKGAFK IPQKAASIVL NGFQSKIIVT DFNFGSHSLL YSTAEVLSHS
510 520 530 540 550
IVDDQDILAL WMPTGEAGEF VVTGAKSGSV SSCGGCSSVG FYPQGDDLLV
560 570 580 590 600
TISQSKGISV LTFDDGLRVL VMDRSFAYEF WVPVLTADPF SPANETVFVQ
610 620 630 640 650
GPSLVRSAAY SSDGSTLDLT GDNNGTSTQI QVFPPKSVSK VTWNGQVITT
660 670 680 690 700
EKTDYDSLIG SLTGPALDSL TLPTISGWKA NDSLPERLPT YDDSWWVAAD
710 720 730 740 750
HMNTSNPTKP ETLPVLYIDD YGYHVGNHLW RGRFEGSASG VYLSVTGGRA
760 770 780 790 800
FGYSAWLNGE FIGSYLGAAY PDTGKLTLSF SNVTVNSNST NILLVLQDNS
810 820 830 840 850
GHDETSEALN PRGINNATLI SSSTKNFTSW KVTGTAGKPD TAIDPVRGIL
860 870 880 890 900
SEGGLYAERL GWHLPDFDDS EWSSASPSNV SSSAGVTFYR TTVSLAIPTG
910 920 930 940 950
LDVAISFTLK ASPSNAALRV LLFVNGYQYG RFSPWIGNQV EFPVPPGILN
960 970 980 990 1000
YDGDNVIGLS VWRQEEGDES MGVNVGWKVT EAFASSFEPI FDAAYLQPGW

TDERLQYA
Length:1,008
Mass (Da):110,685
Last modified:September 11, 2007 - v1
Checksum:i52E5C90A03793D5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476623 Genomic DNA. Translation: EDN99923.1.
RefSeqiXP_001596561.1. XM_001596511.1.

Genome annotation databases

EnsemblFungiiEDN99923; EDN99923; SS1G_02781.
GeneIDi5492844.
KEGGissl:SS1G_02781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476623 Genomic DNA. Translation: EDN99923.1.
RefSeqiXP_001596561.1. XM_001596511.1.

3D structure databases

ProteinModelPortaliA7EBU5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEDN99923; EDN99923; SS1G_02781.
GeneIDi5492844.
KEGGissl:SS1G_02781.

Organism-specific databases

EuPathDBiFungiDB:SS1G_02781.

Phylogenomic databases

InParanoidiA7EBU5.
OMAiYINANTS.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGALB_SCLS1
AccessioniPrimary (citable) accession number: A7EBU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: September 11, 2007
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.