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A7EBU5

- BGALB_SCLS1

UniProt

A7EBU5 - BGALB_SCLS1

Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Binding sitei133 – 1331Substrate; via amide nitrogenBy similarity
    Binding sitei134 – 1341SubstrateBy similarity
    Binding sitei192 – 1921SubstrateBy similarity
    Active sitei193 – 1931Proton donorSequence Analysis
    Binding sitei262 – 2621SubstrateBy similarity
    Active sitei305 – 3051NucleophileSequence Analysis
    Binding sitei370 – 3701SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase B (EC:3.2.1.23)
    Alternative name(s):
    Lactase B
    Gene namesi
    Name:lacB
    ORF Names:SS1G_02781
    OrganismiSclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
    Taxonomic identifieri665079 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeSclerotinia
    ProteomesiUP000001312: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 1008990Probable beta-galactosidase BPRO_0000395233Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi268 ↔ 321By similarity
    Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi594 – 5941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi624 – 6241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi703 – 7031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi782 – 7821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi788 – 7881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi816 – 8161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi826 – 8261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi879 – 8791N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi665079.A7EBU5.

    Structurei

    3D structure databases

    ProteinModelPortaliA7EBU5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    OMAiYINANTS.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A7EBU5-1 [UniParc]FASTAAdd to Basket

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    MLLQSLFAWA LAIGPCIAQN STNSTWPIHN NGLTTQVEWD HYSLMVDGQR     50
    FFLWSGEFHY WRIPVPELWV DVLQKVKAAG FNTFAIYTHW YFHNPNPNTL 100
    DFENAAHNFT KIFDLAKELG MFVVFRPGPY VNAESNAGAF PLWLTTGAYG 150
    ALRNNDERYT EAWTPFWEKV ASIVAPYQFT NGGNVLTYQI ENELGSQWRG 200
    TPSNKVPNLS SVEYMEALEA SARAHGITIP FQANDPNLNS DSWSKDFYDG 250
    YGSVDIYGMD SYPACWTCNL TECDSTNGAY KAFNVIDYYD HFEAISPTQP 300
    SFLPEFQGGS FNPWGGPEGG CPENSPADFA NLFYRNNVGQ RVTAMSLYMI 350
    YGGTNWGWLA APVVATSYDY SSPISENRMI NDKYAETKLF GHFLRVAKDL 400
    TKTDRIGTGK TASTNPNVVY SEIRNPDTNA AFYVTIHKES TVGTREEFYI 450
    NANTSKGAFK IPQKAASIVL NGFQSKIIVT DFNFGSHSLL YSTAEVLSHS 500
    IVDDQDILAL WMPTGEAGEF VVTGAKSGSV SSCGGCSSVG FYPQGDDLLV 550
    TISQSKGISV LTFDDGLRVL VMDRSFAYEF WVPVLTADPF SPANETVFVQ 600
    GPSLVRSAAY SSDGSTLDLT GDNNGTSTQI QVFPPKSVSK VTWNGQVITT 650
    EKTDYDSLIG SLTGPALDSL TLPTISGWKA NDSLPERLPT YDDSWWVAAD 700
    HMNTSNPTKP ETLPVLYIDD YGYHVGNHLW RGRFEGSASG VYLSVTGGRA 750
    FGYSAWLNGE FIGSYLGAAY PDTGKLTLSF SNVTVNSNST NILLVLQDNS 800
    GHDETSEALN PRGINNATLI SSSTKNFTSW KVTGTAGKPD TAIDPVRGIL 850
    SEGGLYAERL GWHLPDFDDS EWSSASPSNV SSSAGVTFYR TTVSLAIPTG 900
    LDVAISFTLK ASPSNAALRV LLFVNGYQYG RFSPWIGNQV EFPVPPGILN 950
    YDGDNVIGLS VWRQEEGDES MGVNVGWKVT EAFASSFEPI FDAAYLQPGW 1000
    TDERLQYA 1008
    Length:1,008
    Mass (Da):110,685
    Last modified:September 11, 2007 - v1
    Checksum:i52E5C90A03793D5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476623 Genomic DNA. Translation: EDN99923.1.
    RefSeqiXP_001596561.1. XM_001596511.1.

    Genome annotation databases

    EnsemblFungiiEDN99923; EDN99923; SS1G_02781.
    GeneIDi5492844.
    KEGGissl:SS1G_02781.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476623 Genomic DNA. Translation: EDN99923.1 .
    RefSeqi XP_001596561.1. XM_001596511.1.

    3D structure databases

    ProteinModelPortali A7EBU5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 665079.A7EBU5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EDN99923 ; EDN99923 ; SS1G_02781 .
    GeneIDi 5492844.
    KEGGi ssl:SS1G_02781.

    Phylogenomic databases

    eggNOGi COG1874.
    OMAi YINANTS.
    OrthoDBi EOG7ZGXBD.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea."
      Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E.
      , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.
      PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 18683 / 1980 / Ss-1.

    Entry informationi

    Entry nameiBGALB_SCLS1
    AccessioniPrimary (citable) accession number: A7EBU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3