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A7E7L8 (PMIP_SCLS1) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial intermediate peptidase

Short name=MIP
EC=3.4.24.59
Alternative name(s):
Octapeptidyl aminopeptidase
Gene names
Name:oct1
ORF Names:SS1G_01296
OrganismSclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum) [Reference proteome]
Taxonomic identifier665079 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeSclerotinia

Protein attributes

Sequence length785 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.

Catalytic activity

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactor

Binds 1 zinc ion By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the peptidase M3 family.

Sequence caution

The sequence EDN96370.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 785758Mitochondrial intermediate peptidase
PRO_0000338594

Regions

Compositional bias283 – 2875Poly-Ala

Sites

Active site5671 By similarity
Metal binding5661Zinc; catalytic By similarity
Metal binding5701Zinc; catalytic By similarity
Metal binding5731Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
A7E7L8 [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 22354DF6D7047D4F

FASTA78587,723
        10         20         30         40         50         60 
MLKAVMPRPW VCSRCVKRQI QSSRGLATAS TQYREPRPVP TDHSAPGAKH DDRTLRQIFD 

        70         80         90        100        110        120 
SPDFWADFSQ SSKQSYYRPG VGLFQNRYLV NPQGFEVFAN TSLRKAQRIV DKVLKASTVE 

       130        140        150        160        170        180 
EYRHVARDLD RLSDLLCRVI DLSDFVRATH PNAAIQAAAS RAYAKMFEYM NILNTTTGLD 

       190        200        210        220        230        240 
KQLEVAMGTP EIVAGWTEEE VVVADILKKD FAKSAIDLPR AQREKFVALS QEISEIGPDF 

       250        260        270        280        290        300 
VDYMTPAKSY LTFESSKLKG MDPVLVREHT TWGQTKIPTI GGAAAAAIRT VQNEEVRREI 

       310        320        330        340        350        360 
FMATRTASRN TVHKLEELMR KRAELAKLSR YESYSQLALG DKMAKSPASV TQFLEALAKD 

       370        380        390        400        410        420 
NNKIVQGEVS ELLKFKFSNP NASSPGLQPW DKDYYMSRIL ASVRSHSRNS DFLSAYFSLG 

       430        440        450        460        470        480 
TVMQGLSRLF TRLYGVRLAP HETMPGETWN SDVRRLDVIS ETDGHVAVLY CDLFSRPGKS 

       490        500        510        520        530        540 
PNPAHFTLRC SREITTAELE EASMLSQNGL FKTDEEAAND GMATSKSSGV LKQLPTIALI 

       550        560        570        580        590        600 
CDFVTMSGKS SRPALLSFNE VQTLFHEMGH AIHSILGRTS LQNVSGTRCA TDFAELPSVL 

       610        620        630        640        650        660 
MEHFAADPSV LSLFARHYET DQQLPYEMVA EKLALDKRFE GSDTENQIIL SMLDLAYHSD 

       670        680        690        700        710        720 
LPLSPTFNST EIYHSLQQKH GALPVDPPGT CWQGFFGHLF GYGSTYYSYL FDRVLARRIW 

       730        740        750        760        770        780 
QVVFQGGEAG GSVHRGNGEK MKEEVLKWGG GRDPWKCLAG VLDDGRVENG DEKAMAIVGS 


WGVKE 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH476622 Genomic DNA. Translation: EDN96370.1. Different initiation.
RefSeqXP_001597102.1. XM_001597052.1.

3D structure databases

ProteinModelPortalA7E7L8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING665079.A7E7L8.

Protein family/group databases

MEROPSM03.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEDN96370; EDN96370; SS1G_01296.
GeneID5493674.
KEGGssl:SS1G_01296.

Phylogenomic databases

eggNOGCOG0339.
KOK01410.
OrthoDBEOG71GB4R.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
3.40.390.10. 2 hits.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMIP_SCLS1
AccessionPrimary (citable) accession number: A7E7L8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 13, 2013
This is version 37 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries