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Protein

Neutrophil cytosol factor 2

Gene

Ncf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity

GO - Biological processi

  • aging Source: RGD
  • cellular response to hormone stimulus Source: RGD
  • positive regulation of blood pressure Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • response to activity Source: RGD
  • response to drug Source: RGD
  • response to glucose Source: RGD
  • response to hyperoxia Source: RGD
  • response to laminar fluid shear stress Source: RGD
  • response to lipid Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to progesterone Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 2
Short name:
NCF-2
Alternative name(s):
Neutrophil NADPH oxidase factor 2
Gene namesi
Name:Ncf2Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1309424. Ncf2.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Neutrophil cytosol factor 2PRO_0000312228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei225 – 2251PhosphothreonineBy similarity
Modified residuei316 – 3161PhosphoserineBy similarity
Modified residuei400 – 4001PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA7E3N2.
PRIDEiA7E3N2.

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060733.

Structurei

3D structure databases

ProteinModelPortaliA7E3N2.
SMRiA7E3N2. Positions 1-167, 234-289, 351-429, 456-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 7034TPR 1Sequence analysisAdd
BLAST
Repeati72 – 10433TPR 2Sequence analysisAdd
BLAST
Repeati119 – 15234TPR 3Sequence analysisAdd
BLAST
Domaini232 – 29160SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini352 – 43079PB1PROSITE-ProRule annotationAdd
BLAST
Domaini458 – 51760SH3 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation
Contains 3 TPR repeats.Sequence analysis

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
HOGENOMiHOG000237312.
HOVERGENiHBG001521.
InParanoidiA7E3N2.
KOiK08010.
OMAiYSQVRDM.
OrthoDBiEOG7G1V62.
PhylomeDBiA7E3N2.
TreeFamiTF329087.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7E3N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLAEAIRLW NEGVQAADKK DWKGALEAFS EVQDPHSRIC FNIGCMYTIL
60 70 80 90 100
DNLQEAEQAF TKSINRDKHL AVAYFQRGML YYSMEKYRPA SVGRKAALLF
110 120 130 140 150
LGSYNLVARI IVGYPLSPGK VLYNIALMHA KKEEWKKAEE QLALATNMKS
160 170 180 190 200
EPRHSKIDKA MESIWKRCPT SHLPLDPPQV TMALWFEEGG VGKRSVVASV
210 220 230 240 250
VHQDNFSGFA PLQPQSAEPP PRPKTPEIFR ALEGEAHRVL FGFVPETPEE
260 270 280 290 300
LQVMPGNIVF VLKKGSDNWA TVMFNGQKGL VPCNYLEPVE LRIHPQSQPQ
310 320 330 340 350
EDTSLESDIP PPPNSSPPER LQLSPGWCQQ LGPLRCPPFL LHQEVKRSVP
360 370 380 390 400
MPYMLKVHYK YTVVMETQLG LPYSQLRNMV SKKLELLPEH TKLSYQRRDS
410 420 430 440 450
PELLLLSEES MKDAWAQVKN YCLTLWCEHT VGDQGFVDEP KEKENSDADN
460 470 480 490 500
RTTEPQPKEG TQVVAIFSYD ATQPEDLEFV EGDVILVLSH VNEEWLEGEC
510 520
KGKIGIFPKA FVEGCAAKNL EGTPREV
Length:527
Mass (Da):59,573
Last modified:September 11, 2007 - v1
Checksum:iE025D50A0BA222E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03084702 Genomic DNA. No translation available.
AABR03085295 Genomic DNA. No translation available.
AABR03088578 Genomic DNA. No translation available.
BR000294 mRNA. Translation: FAA00361.1.
RefSeqiNP_001094454.1. NM_001100984.1.
UniGeneiRn.162331.

Genome annotation databases

GeneIDi364018.
KEGGirno:364018.
UCSCiRGD:1309424. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03084702 Genomic DNA. No translation available.
AABR03085295 Genomic DNA. No translation available.
AABR03088578 Genomic DNA. No translation available.
BR000294 mRNA. Translation: FAA00361.1.
RefSeqiNP_001094454.1. NM_001100984.1.
UniGeneiRn.162331.

3D structure databases

ProteinModelPortaliA7E3N2.
SMRiA7E3N2. Positions 1-167, 234-289, 351-429, 456-516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060733.

Proteomic databases

PaxDbiA7E3N2.
PRIDEiA7E3N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi364018.
KEGGirno:364018.
UCSCiRGD:1309424. rat.

Organism-specific databases

CTDi4688.
RGDi1309424. Ncf2.

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
HOGENOMiHOG000237312.
HOVERGENiHBG001521.
InParanoidiA7E3N2.
KOiK08010.
OMAiYSQVRDM.
OrthoDBiEOG7G1V62.
PhylomeDBiA7E3N2.
TreeFamiTF329087.

Enzyme and pathway databases

ReactomeiR-RNO-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-5668599. RHO GTPases Activate NADPH Oxidases.

Miscellaneous databases

NextBioi684661.
PROiA7E3N2.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway1 Publication.
  2. "Molecular evolution of the reactive oxygen-generating NADPH oxidase (Nox/Duox) family of enzymes."
    Kawahara B.T., Quinn M.T., Lambeth J.D.
    BMC Evol. Biol. 7:109-109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiNCF2_RAT
AccessioniPrimary (citable) accession number: A7E3N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: September 11, 2007
Last modified: May 11, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.