A7DZP8 (GPDM_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial
Short name=GPD-M
Short name=GPDH-M
EC=1.1.5.3

Alternative name(s):
mGPD

Gene names

Name:

GPD2

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD By similarity.
Enzyme regulation	Calcium-binding enhance the activity of the enzyme By similarity.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (aerobic route): step 1/1.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	Calcium FAD Flavoprotein Metal-binding
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	glycerol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Mitochondrion By similarity
	Chain	43 – 727	685	Glycerol-3-phosphate dehydrogenase, mitochondrial		PRO_0000355968
Regions
	Domain	623 – 658	36	EF-hand 1
	Domain	659 – 694	36	EF-hand 2
	Nucleotide binding	71 – 99	29	FAD Potential
	Calcium binding	672 – 683	12	Potential
Amino acid modifications
	Modified residue	600	1	Phosphotyrosine By similarity
	Modified residue	601	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7DZP8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 89C18A4C8D0205DA	FASTA	727	80,857
	10 20 30 40 50 60 MAFQKAVKRT VLVCGGALAT VLGLSQCSHY RRKQVNLACL KAAGCHTEPV NREPPSREAQ 70 80 90 100 110 120 LLTLQNTSEF DILVIGGGAT GSGCALDAVT RGLKTALVER DDFSSGTSSR STKLIHGGVR 130 140 150 160 170 180 YLQKAIMKLD VEQYRMVKEA LHERANLLEI APHLSAPLPI MLPIYKWWQL PYYWVGIKLY 190 200 210 220 230 240 DLVAGSNCLK SSYVLSKSRA LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG 250 260 270 280 290 300 AATANYREVV SLLKKTDPET GKERVSGARC KDVLTGLEFD VRAKCVINAT GPFTDSVRKM 310 320 330 340 350 360 DDNKAPAICQ PSAGVHIVMP GYYSPESMGL LDPATSDGRV IFFLPWENMT IAGTTDSPTK 370 380 390 400 410 420 NTHHPIPSEE DINFILNEVR NYLSCDVEVR RGDVLAAWSG IRPLVIDPKS ADTQSISRNH 430 440 450 460 470 480 VVDVSESGLI TIAGGKWTTY RSMAEDTVDT AIKVHNLKAG PCRTVGLFLQ GGKDWSPTLY 490 500 510 520 530 540 IRLVQDYGLE SEVAQHLATT YGDKAFEVAK MAKVTGKRWP IVGVRLVSEF PYIEAEVKYG 550 560 570 580 590 600 IKEYACTAVD MISRRTRLAF LNIQAAEEAL PRIVELMGRE LDWSEIRKQA ELETATKFLY 610 620 630 640 650 660 YEMGSKSRSE QLTDRTEISL RPSDIERYTK RFHKFDADEK GFITIVDVQR VLENINVKID 670 680 690 700 710 720 ENTLHEILSE VDLNKNGQVE LNEFLQLMSA IQKGRVSGSR LAILMKTAEE NLDRRVPIPV DRSCGGL

References

[1]	"Hamster sperm capacitation: role of FAD linked glycerol-3-phosphate dehydrogenase." Venkatesh K., Shivaji S. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774032 mRNA. Translation: CAO79918.1.
3D structure databases
ProteinModelPortal	A7DZP8.
ModBase	Search...
Proteomic databases
PRIDE	A7DZP8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG005897.
Enzyme and pathway databases
UniPathway	UPA00618; UER00674.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR006076. FAD-dep_OxRdtase. IPR000447. G3P_DH_FAD-dep. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF13499. EF_hand_5. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDM_MESAU

Accession

Primary (citable) accession number: A7DZP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program