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A7BJC4 (IOLA_BACNA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylmalonate semialdehyde dehydrogenase [acylating]

Short name=MMSA dehydrogenase
Short name=MMSDH
Short name=MSDH
EC=1.2.1.27
Alternative name(s):
Malonate semialdehyde dehydrogenase [acetylating]
Short name=MSA dehydrogenase
EC=1.2.1.18
Gene names
Name:iolA
OrganismBacillus subtilis subsp. natto
Taxonomic identifier86029 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity. HAMAP-Rule MF_01670

Catalytic activity

3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP-Rule MF_01670

2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP-Rule MF_01670

Pathway

Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP-Rule MF_01670

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01670

Sequence similarities

Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Sequence caution

The sequence BAF74776.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487Methylmalonate semialdehyde dehydrogenase [acylating] HAMAP-Rule MF_01670
PRO_0000352332

Regions

Nucleotide binding176 – 1805NAD By similarity

Sites

Active site2841Nucleophile By similarity
Binding site3821NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A7BJC4 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: ABB479387DF2B49C

FASTA48753,461
        10         20         30         40         50         60 
MAEIRKLKNY INGEWVESKT DQYEDVVNPA TKEVLCQVPI STKEDIDYAA QTAAEAFETW 

        70         80         90        100        110        120 
SKVAVPRRAR ILFNFQQLLS QHKEELAHLI TIENGKNTKE ALGEVGRGIE NVEFAAGAPS 

       130        140        150        160        170        180 
LMMGDSLASI ATDVEAANYR YPIGVVGGIA PFNFPMMVPC WMFPMAIALG NTFILKPSER 

       190        200        210        220        230        240 
TPLLTEKLVE LFEKAGLPKG VFNVVYGAHD VVNGILEHPE IKAISFVGSK PVGEYVYKKG 

       250        260        270        280        290        300 
SEHLKRVQSL TGAKNHTIVL NDANLEDTVT NIVGAAFGSA GERCMACAVV TVEEGIADEF 

       310        320        330        340        350        360 
MAKLQEKVAD IKIGNGLDDG VFLGPVIRED NKKRTLSYIE KGLEEGARLV CDGRENVSDD 

       370        380        390        400        410        420 
GYFVGPTIFD NVTTEMTIWK DEIFAPVLSV IRVKNLKEAI EIANKSEFAN GACLFTSNSN 

       430        440        450        460        470        480 
AIRYFRENID AGMLGINLGV PAPMAFFPFS GWKSSFFGTL HANGKDSVDF YTRKKVVTAR 


FPAPDFN 

« Hide

References

[1]"Determination and characterization of IS4Bsu1-insertion loci and identification of a new insertion sequence element of the IS256 family in a natto starter."
Kimura K., Itoh Y.
Biosci. Biotechnol. Biochem. 71:2458-2464(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Miyagino.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB304464 Genomic DNA. Translation: BAF74776.1. Different initiation.

3D structure databases

ProteinModelPortalA7BJC4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00076; UER00148.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPMF_01670. IolA.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
IPR023510. MeMal-semiAld_DH_GmP_bac.
[Graphical view]
PANTHERPTHR11699:SF27. PTHR11699:SF27. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01722. MMSDH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIOLA_BACNA
AccessionPrimary (citable) accession number: A7BJC4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 14, 2008
Last modified: February 19, 2014
This is version 36 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways