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Protein

Methylmalonate semialdehyde dehydrogenase [acylating]

Gene

iolA

Organism
Bacillus subtilis subsp. natto
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively.UniRule annotation

Catalytic activityi

3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H.UniRule annotation
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH.UniRule annotation

Pathway: myo-inositol degradation into acetyl-CoA

This protein is involved in step 7 of the subpathway that synthesizes acetyl-CoA from myo-inositol.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. 5-deoxy-glucuronate isomerase (iolB)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. Methylmalonate semialdehyde dehydrogenase [acylating] (iolA)
This subpathway is part of the pathway myo-inositol degradation into acetyl-CoA, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from myo-inositol, the pathway myo-inositol degradation into acetyl-CoA and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei284 – 2841NucleophileUniRule annotation
Binding sitei382 – 3821NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi176 – 1805NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00076; UER00148.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylmalonate semialdehyde dehydrogenase [acylating]UniRule annotation (EC:1.2.1.27UniRule annotation)
Short name:
MMSA dehydrogenaseUniRule annotation
Short name:
MMSDHUniRule annotation
Short name:
MSDHUniRule annotation
Alternative name(s):
Malonate semialdehyde dehydrogenase [acetylating]UniRule annotation (EC:1.2.1.18UniRule annotation)
Short name:
MSA dehydrogenaseUniRule annotation
Gene namesi
Name:iolAUniRule annotation
OrganismiBacillus subtilis subsp. natto
Taxonomic identifieri86029 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Methylmalonate semialdehyde dehydrogenase [acylating]PRO_0000352332Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA7BJC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family. IolA subfamily.UniRule annotation

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_01670. IolA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
IPR023510. MeMal-semiAld_DH_GmP_bac.
[Graphical view]
PANTHERiPTHR11699:SF27. PTHR11699:SF27. 1 hit.
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01722. MMSDH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7BJC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEIRKLKNY INGEWVESKT DQYEDVVNPA TKEVLCQVPI STKEDIDYAA
60 70 80 90 100
QTAAEAFETW SKVAVPRRAR ILFNFQQLLS QHKEELAHLI TIENGKNTKE
110 120 130 140 150
ALGEVGRGIE NVEFAAGAPS LMMGDSLASI ATDVEAANYR YPIGVVGGIA
160 170 180 190 200
PFNFPMMVPC WMFPMAIALG NTFILKPSER TPLLTEKLVE LFEKAGLPKG
210 220 230 240 250
VFNVVYGAHD VVNGILEHPE IKAISFVGSK PVGEYVYKKG SEHLKRVQSL
260 270 280 290 300
TGAKNHTIVL NDANLEDTVT NIVGAAFGSA GERCMACAVV TVEEGIADEF
310 320 330 340 350
MAKLQEKVAD IKIGNGLDDG VFLGPVIRED NKKRTLSYIE KGLEEGARLV
360 370 380 390 400
CDGRENVSDD GYFVGPTIFD NVTTEMTIWK DEIFAPVLSV IRVKNLKEAI
410 420 430 440 450
EIANKSEFAN GACLFTSNSN AIRYFRENID AGMLGINLGV PAPMAFFPFS
460 470 480
GWKSSFFGTL HANGKDSVDF YTRKKVVTAR FPAPDFN
Length:487
Mass (Da):53,461
Last modified:October 14, 2008 - v2
Checksum:iABB479387DF2B49C
GO

Sequence cautioni

The sequence BAF74776.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB304464 Genomic DNA. Translation: BAF74776.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB304464 Genomic DNA. Translation: BAF74776.1. Different initiation.

3D structure databases

ProteinModelPortaliA7BJC4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00076; UER00148.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_01670. IolA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
IPR023510. MeMal-semiAld_DH_GmP_bac.
[Graphical view]
PANTHERiPTHR11699:SF27. PTHR11699:SF27. 1 hit.
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01722. MMSDH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Determination and characterization of IS4Bsu1-insertion loci and identification of a new insertion sequence element of the IS256 family in a natto starter."
    Kimura K., Itoh Y.
    Biosci. Biotechnol. Biochem. 71:2458-2464(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Miyagino.

Entry informationi

Entry nameiIOLA_BACNA
AccessioniPrimary (citable) accession number: A7BJC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 14, 2008
Last modified: October 1, 2014
This is version 38 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.