A7A0P0 (SDC25_YEAS7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide exchange factor SDC25

Gene names

Name:	SDC25
Synonyms:	SCD25
ORF Names:	SCY_3564

Organism

Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) [Complete proteome]

Taxonomic identifier

307796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Promotes the exchange of Ras-bound GDP by GTP By similarity.
Miscellaneous	Suppresses the CDC25-5 mutation in yeast (restores cAMP level) and has similar functions as CDC25.
Sequence similarities	Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. Contains 1 SH3 domain.
Sequence caution	The sequence EDN59531.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	SH3 domain
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1252	1252	Guanine nucleotide exchange factor SDC25		PRO_0000393439
Regions
	Domain	26 – 97	72	SH3
	Domain	782 – 914	133	N-terminal Ras-GEF
	Domain	952 – 1199	248	Ras-GEF

Sequences

	Sequence		Length	Mass (Da)
	A7A0P0 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: 076BCCE7C1F1EBE8	FASTA	1,252	145,070
	10 20 30 40 50 60 MSCTASYAGM TTPVKDKEGH GIPCLQPIDV VECTYQYFTK SRNKLSLRVG DLIYVLTKGS 70 80 90 100 110 120 NGWWDGVLIR HSANNNNNSL ILDRGWFPPS FTRSILNELH GVPDIGNELE IFQAGLNLKL 130 140 150 160 170 180 ELSSNPVILS LEDFLDCCRD IEFKEQLAWS PTPVHERKGC CELLYYNQDL DVYCRTLPYL 190 200 210 220 230 240 PQNQVETVND YSSFPAISKI AGKKMPITSS PDLFYLNDCD VVYWYDLTRL VCHYVNLTER 250 260 270 280 290 300 DLLANEREKF LTSLDLLTAQ ITCVYMLFRN LRLVEDSFKK TLKKLIYTLS RFSINANIWF 310 320 330 340 350 360 HSTPFEEREA IASQKDPERR SPLLQSILGT FQKFHFLLRL LHFLSNPNEL TILPQLTPRF 370 380 390 400 410 420 FKDSFNTISW NNPFLRKHLN QHMSHDLPRQ MIKAVAGASG IVAENNDEIP ASKQGTSCSS 430 440 450 460 470 480 ETSHHSPSAP FQRRRRGTIF SNVPGSSDES DTIWSKRKKP YPLNEETLSL VRARKEQLDA 490 500 510 520 530 540 KLKQMIKSAN EYLSNTANFS KMLNFEMNFK TYEEVSGTIP IIDILENLDL TIYLNLRELG 550 560 570 580 590 600 DENRVFDEDV AIDDEDKEFL KHSLSSLSYI LSDYFNMKQY FHDVVVKFII VAQHLTLEDP 610 620 630 640 650 660 FVFSPMQNDL PTGYYEPMKP SSLNLDNAKD KKNGSQNTDI QEEEDEYEPD PDSLILFHNL 670 680 690 700 710 720 INQDSDFNDL KFFNLAHVFK KSCDDYFDVL KLSIEFVNRL ILERENLLNY AARMMKNNIT 730 740 750 760 770 780 ELLLRGEEGY GSYDGGETAE KSDTNAVYAD SDTKDNDEWR DSQVKLPRYL QREYDSELIW 790 800 810 820 830 840 GSNNRIKGGS KHALISYLTD NEKKDLFFNI TFLITFRSIF TTTEFLSYLI SQYNLDPPED 850 860 870 880 890 900 LCFEEYNEWV TKKLIPVKCR VVEIMTTFFK QYWFPGYDEP DLATLNLDYF AQVAIKENIT 910 920 930 940 950 960 GSVELLKEVN QKFKHGNMQE ATAPMKTLDQ QICQEHYWGT LYSTTESILA VDPVLFATQL 970 980 990 1000 1010 1020 TILEHEIYCE ITIFDCLQKI WKNKYTKSYG ASPGLNEFIS FANKLTNFIS YSIVKEADKS 1030 1040 1050 1060 1070 1080 KRAKLLSHFI FIAEYCRKFN NFSSMTAIIS ALYSSSIYRL EKTWQAVIPQ TRDLLQSLDK 1090 1100 1110 1120 1130 1140 LMDPKKNFIN YRSELKSLHS APCVPFFGVY LSDLTFTDSG NPDYLVLEHG LKGVHDEKKY 1150 1160 1170 1180 1190 1200 INFNKRSRLV DILQEIIYFK KTHYDFTKDR TVIECISNSL ENIPHIEKQY QLSLIIEPKP 1210 1220 1230 1240 1250 RKKVVPNSNS NNKSQEKSRD DQTDEGKTST KKDRFSKFQL HKTKKKAPKV SK

References

[1]

"Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789." Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z., Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X., Jia P., Luedi P., Oefner P.J., David L. , Dietrich F.S., Li Y., Davis R.W., Steinmetz L.M. Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJM789.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFW02000167 Genomic DNA. Translation: EDN59531.1. Different initiation.
3D structure databases
ProteinModelPortal	A7A0P0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.840.10. 1 hit.
InterPro	IPR000651. Ras-like_Gua-exchang_fac_N. IPR019804. Ras_G-nucl-exch_fac_CS. IPR008937. Ras_GEF. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. IPR001452. SH3_domain. [Graphical view]
PANTHER	PTHR23113. PTHR23113. 1 hit.
Pfam	PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00720. RASGEF. 1 hit. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SDC25_YEAS7

Accession

Primary (citable) accession number: A7A0P0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	March 6, 2013
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program