A6ZZV7 (LAC1_YEAS7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingosine N-acyltransferase LAC1
EC=2.3.1.24

Gene names

Name:	LAC1
ORF Names:	SCY_3367

Organism

Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) [Complete proteome]

Taxonomic identifier

307796 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Redundant with LAG1. Facilitates ER-to-Golgi transport of GPI-anchored proteins By similarity.
Catalytic activity	Acyl-CoA + sphingosine = CoA + N-acylsphingosine.
Subunit structure	Composed of the ceramide synthase complex composed of at least LAC1, LAG1 and LIP1 By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the sphingosine N-acyltransferase family. Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Ontologies

Keywords
Biological process	Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sphingosine N-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Sphingosine N-acyltransferase LAC1		PRO_0000308912
Regions
	Topological domain	1 – 81	81	Cytoplasmic Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Topological domain	103 – 134	32	Lumenal Potential
	Transmembrane	135 – 155	21	Helical; Potential
	Topological domain	156 – 172	17	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Topological domain	194 – 221	28	Lumenal Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Topological domain	243 – 259	17	Cytoplasmic Potential
	Transmembrane	260 – 280	21	Helical; Potential
	Topological domain	281 – 296	16	Lumenal Potential
	Transmembrane	297 – 317	21	Helical; Potential
	Topological domain	318 – 355	38	Cytoplasmic Potential
	Transmembrane	356 – 376	21	Helical; Potential
	Topological domain	377 – 418	42	Lumenal Potential
	Domain	168 – 384	217	TLC
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6ZZV7 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 7691BA623AC0460A	FASTA	418	48,992
	10 20 30 40 50 60 MSTIKPSPSN NNLKVRSRPR RKSSIGKIDL GDTVPSLGTM FETKESKTAA KRRMQRLSEA 70 80 90 100 110 120 TKNDSDLVKK IWFSFREISY RHAWIAPLMI LIAVYSAYFT SGNTTKTNVL HRFVAVSYQI 130 140 150 160 170 180 GDTNAYGKGI NDLCFVFYYM IFFTFLREFL MDVVIRPFAI RLHVTSKHRI KRIMEQMYAI 190 200 210 220 230 240 FYTGVSGPFG IYCMYHSDLW FFNTKAMYRT YPDFTNPFLF KVFYLGQAAF WAQQACILVL 250 260 270 280 290 300 QLEKPRKDHN ELTFHHIVTL LLIWSSYVFH FTKMGLPIYI TMDVSDFLLS FSKTLNYLDS 310 320 330 340 350 360 GLAFFSFAIF VVAWIYLRHY INLKILWSVL TQFRTEGNYV LNFATQQYKC WISLPIVFVL 370 380 390 400 410 IGALQLVNLY WLFLIFRVLY RILWRGILKD DRSDSESDEE SDESSTTPTD STPTKKDI

References

[1]

"Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789." Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z., Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X., Jia P., Luedi P., Oefner P.J., David L. , Dietrich F.S., Li Y., Davis R.W., Steinmetz L.M. Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007) [PubMed: 17652520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJM789.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFW02000152 Genomic DNA. Translation: EDN59900.1.
3D structure databases
ProteinModelPortal	A6ZZV7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.24. 984.
Family and domain databases
InterPro	IPR016439. Longevity_assurance_LAG1_LAC1. IPR006634. TLC-dom. IPR013599. TRAM1. [Graphical view]
Pfam	PF08390. TRAM1. 1 hit. PF03798. TRAM_LAG1_CLN8. 1 hit. [Graphical view]
PIRSF	PIRSF005225. LAG1_LAC1. 1 hit.
SMART	SM00724. TLC. 1 hit. [Graphical view]
PROSITE	PS50922. TLC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC1_YEAS7

Accession

Primary (citable) accession number: A6ZZV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	September 11, 2007
Last modified:	May 31, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program