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Protein

Mitochondrial intermediate peptidase

Gene

OCT1

Organism
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane. Cleaves precursor proteins of respiratory components, including subunits of the electron transport chain and tricarboxylic acid cycle enzymes, and components of the mitochondrial genetic machinery, including ribosomal proteins, translation factors, and proteins required for mitochondrial DNA metabolism.

Miscellaneous

Present with 2690 molecules/cell in log phase SD medium.

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Enzyme regulationi

Stimulated by Fe2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi558Zinc; catalyticPROSITE-ProRule annotation1
Active sitei559PROSITE-ProRule annotation1
Metal bindingi562Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi565Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Alternative name(s):
Octapeptidyl aminopeptidase
Gene namesi
Name:OCT1
ORF Names:SCY_3247
OrganismiSaccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Taxonomic identifieri307796 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000007060 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37MitochondrionSequence analysisAdd BLAST37
ChainiPRO_000034008438 – 772Mitochondrial intermediate peptidaseAdd BLAST735

Proteomic databases

PRIDEiA6ZZI7

Structurei

3D structure databases

ProteinModelPortaliA6ZZI7
SMRiA6ZZI7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

OrthoDBiEOG092C0JU2

Family and domain databases

CDDicd06457 M3A_MIP, 1 hit
Gene3Di1.10.1370.10, 2 hits
InterProiView protein in InterPro
IPR033851 M3A_MIP
IPR024077 Neurolysin/TOP_dom2
IPR001567 Pept_M3A_M3B
PANTHERiPTHR11804:SF5 PTHR11804:SF5, 1 hit
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6ZZI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTIILKAG SNASIPSLSR QNKLLRFFAT AGAVSRTSPG SIKKIFDDNS
60 70 80 90 100
YWRNINGQDA NNSKISQYLF KKNKTGLFKN PYLTSPDGLR KFSQVSLQQA
110 120 130 140 150
QELLDKMRND FSESGKLTYI MNLDRLSDTL CRVIDLCEFI RSTHPDDAFV
160 170 180 190 200
RAAQDCHEQM FEFMNVLNTD VSLCNMLKSV LNNPEVSSKL SAEELKVGKI
210 220 230 240 250
LLDDFEKSGI YMNPDVREKF IQLSQEISLV GQEFINHTDY PGSNSVKIPC
260 270 280 290 300
KDLDNSKVST FLLKQLNKDV KGQNYKVPTF GYAAYALLKS CENEMVRKKL
310 320 330 340 350
WTALHSCSDK QVKRLSHLIK LRAILANLMH KTSYAEYQLE GKMAKNPKDV
360 370 380 390 400
QDFILTLMNN TIEKTANELK FIAELKAKDL KKPLTTNTDE ILKLVRPWDR
410 420 430 440 450
DYYTGKYFQL NPSNSPSAKE ISYYFTLGNV IQGLSDLFQQ IYGIRLEPAI
460 470 480 490 500
TDEGETWSPD VRRLNVISEE EGIIGIIYCD LFERNGKTSN PAHFTVCCSR
510 520 530 540 550
QIYPSETDFS TIQVGENPDG TYFQLPVISL VCNFSPILIA SKKSLCFLQL
560 570 580 590 600
SEVETLFHEM GHAMHSMLGR THMQNISGTR CATDFVELPS ILMEHFAKDI
610 620 630 640 650
RILTKIGKHY GTGETIQADM LQRFMKSTNF LQNCETYSQA KMAMLDQSFH
660 670 680 690 700
DEKIISDIDN FDVVENYQAL ERRLKVLVDD QSNWCGRFGH LFGYGATYYS
710 720 730 740 750
YLFDRTIASK IWYALFEDDP YSRKNGDKFK KHLLKWGGLK DPWKCIADVL
760 770
ECPMLEKGGS DAMEFIAQSH KS
Length:772
Mass (Da):88,190
Last modified:September 11, 2007 - v1
Checksum:i4A5E12AEE44397D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFW02000151 Genomic DNA Translation: EDN60035.1

Genome annotation databases

EnsemblFungiiEDN60035; EDN60035; SCY_3247

Similar proteinsi

Entry informationi

Entry nameiPMIP_YEAS7
AccessioniPrimary (citable) accession number: A6ZZI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 11, 2007
Last modified: March 28, 2018
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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