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A6ZXD2 (TRMB_YEAS7) Reviewed, UniProtKB/Swiss-Prot

Last modified October 3, 2012. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
ORF Names:SCY_0720
OrganismSaccharomyces cerevisiae (strain YJM789) (Baker's yeast) [Complete proteome]
Taxonomic identifier307796 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methyltransferase that catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity By similarity.

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis.

Subunit structure

Forms a complex with TRM82 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286tRNA (guanine-N(7)-)-methyltransferase
PRO_0000370608

Regions

Region126 – 1272S-adenosyl-L-methionine binding By similarity
Region161 – 1622S-adenosyl-L-methionine binding By similarity
Region259 – 2613S-adenosyl-L-methionine binding By similarity

Sites

Active site1841 By similarity
Binding site1031S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1811S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue71Phosphoserine By similarity
Modified residue371Phosphoserine By similarity
Modified residue591Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
A6ZXD2 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 029406EC502C85B7

FASTA28633,391
        10         20         30         40         50         60 
MKAKPLSQDP GSKRYAYRIN KEENRKELKH VKINESSLVQ EGQKIDLPKK RYYRQRAHSN 

        70         80         90        100        110        120 
PFSDHQLEYP VSPQDMDWSK LYPYYKNAEN GQMTKKVTIA DIGCGFGGLM IDLSPAFPED 

       130        140        150        160        170        180 
LILGMEIRVQ VTNYVEDRII ALRNNTASKH GFQNINVLRG NAMKFLPNFF EKGQLSKMFF 

       190        200        210        220        230        240 
CFPDPHFKQR KHKARIITNT LLSEYAYVLK EGGVVYTITD VKDLHEWMVK HLEEHPLFER 

       250        260        270        280 
LSKEWEENDE CVQIMRNATE EGKKVERKKG DKFVACFTRL PTPAIL

« Hide

References

[1]"Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789."
Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z., Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X., Jia P., Luedi P., Oefner P.J., David L. expand/collapse author list , Dietrich F.S., Li Y., Davis R.W., Steinmetz L.M.
Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YJM789.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFW02000145 Genomic DNA. Translation: EDN60162.1.

3D structure databases

ProteinModelPortalA6ZXD2.
SMRA6ZXD2. Positions 60-286.
ModBaseSearch...

Proteomic databases

PRIDEA6ZXD2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_YEAS7
AccessionPrimary (citable) accession number: A6ZXD2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 11, 2007
Last modified: October 3, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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