Reviewed, UniProtKB/Swiss-Prot A6ZTP2 (ATG15_YEAS7)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15
    Cytoplasm to vacuole targeting protein 17

Gene names

Name:	ATG15
Synonyms:	AUT5, CVT17
ORF Names:	SCY_0635

Organism

Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) [Complete proteome]

Taxonomic identifier

307796 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Putative lipase ATG15		PRO_0000317974
Regions
	Topological domain	1 – 14	14	Cytoplasmic By similarity
	Transmembrane	15 – 35	21	Signal-anchor for type II membrane protein
	Topological domain	36 – 520	485	Lumenal By similarity
Sites
	Active site	332	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	208	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6ZTP2-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: FAAA49BB1B5A9845	FASTA	520	58,423
	10 20 30 40 50 60 MLHKSPSRKR FASPLHLGCI LTLTVLCLIA YYFALPDYLS VGKSSSRGAM DQKSDGTFRL 70 80 90 100 110 120 KSIYRHGVGA NHRLHQRLEV TPEVISAAGM LYQETTTQGQ DFEDQEPLWT TNAEYATTNP 130 140 150 160 170 180 FDFEFELRRM PLLMKRMKER DPEFIESYIY GETYMTEEEE HAMWIDDDIV APNITDRGTV 190 200 210 220 230 240 VSLALMSSNA YVRIPQTGDW RNVTEPWNET EPEDFGWDGD GIRGHVFYNE VENIVVLSIK 250 260 270 280 290 300 GTSAQGLPGS GEDETTGNDK INDNLLFSCC CARVSYLWTT VCDCYVKSYT CDESCLEKEL 310 320 330 340 350 360 RRKDRFYSAV VDIYKGVLKE YPDAAIWVTG HSLGGALASL LGRTFGLPAV AFESPGELLP 370 380 390 400 410 420 SKRLHLPFPP GLPSYMEGIW HFGHNADPIF MGTCNGASSS CSLVGYAMET ACHTGRVCVY 430 440 450 460 470 480 DVVNDKGWSV NMFNHRIHKV IDEVLLGYEQ AAKCVEPEPC VDCYNWKFIP SRDWESSSRL 490 500 510 520 ITKTKSHAAP TTTTRTTATT TSSSTCVGRN WLGFCTKYEL

References

[1]

"Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789." Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z., Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X., Jia P., Luedi P., Oefner P.J., David L. , Dietrich F.S., Li Y., Davis R.W., Steinmetz L.M. Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007) [PubMed: 17652520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFW02000089 Genomic DNA. Translation: EDN62181.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_YEAS7

Accession

Primary (citable) accession number: A6ZTP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)