A6ZSP9 (LAG1_YEAS7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingosine N-acyltransferase LAG1
EC=2.3.1.24

Alternative name(s):
Longevity assurance factor 1
Longevity assurance protein 1

Gene names

Name:	LAG1
ORF Names:	SCY_2383

Organism

Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) [Complete proteome]

Taxonomic identifier

307796 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Redundant to LAC1. Facilites ER-to-Golgi transport of GPI-anchored proteins. Involved in the aging process By similarity.
Catalytic activity	Acyl-CoA + sphingosine = CoA + N-acylsphingosine.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the sphingosine N-acyltransferase family. Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Ontologies

Keywords
Biological process	Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sphingosine N-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Sphingosine N-acyltransferase LAG1		PRO_0000308913
Regions
	Topological domain	1 – 81	81	Cytoplasmic By similarity
	Transmembrane	82 – 102	21	Helical; Potential
	Topological domain	103 – 134	32	Lumenal By similarity
	Transmembrane	135 – 155	21	Helical; Potential
	Topological domain	156 – 176	21	Cytoplasmic By similarity
	Transmembrane	177 – 197	21	Helical; Potential
	Topological domain	198 – 211	14	Lumenal By similarity
	Transmembrane	212 – 232	21	Helical; Potential
	Topological domain	233 – 251	19	Cytoplasmic By similarity
	Transmembrane	252 – 272	21	Helical; Potential
	Topological domain	273 – 296	24	Lumenal By similarity
	Transmembrane	297 – 317	21	Helical; Potential
	Topological domain	318 – 355	38	Cytoplasmic By similarity
	Transmembrane	356 – 376	21	Helical; Potential
	Topological domain	377 – 411	35	Lumenal By similarity
	Domain	168 – 384	217	TLC
Amino acid modifications
	Modified residue	8	1	Phosphoserine By similarity
	Modified residue	23	1	Phosphoserine By similarity
	Modified residue	24	1	Phosphoserine By similarity
	Glycosylation	103	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6ZSP9 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: C3A2847B9613EB9E	FASTA	411	48,470
	10 20 30 40 50 60 MTSATDKSID RLVVNAKTRR RNSSVGKIDL GDTVPGFAAM PESAASKNEA KKRMKALTGD 70 80 90 100 110 120 SKKDSDLLWK VWFSYREMNY RHSWLTPFFI LVCVYSAYFL SGNRTESNPL HMFVAISYQV 130 140 150 160 170 180 DGTDSYAKGI KDLSFVFFYM IFFTFLREFL MDVVIRPFTV YLNVTSEHRQ KRMLEQMYAI 190 200 210 220 230 240 FYCGVSGPFG LYIMYHSDLW LFKTKPMYRT YPDITNPFLF KIFYLGQAAF WAQQACVLVL 250 260 270 280 290 300 QLEKPRKDYK ELVFHHIVTL LLIWSSYVFH FTKMGLAIYI TMDVSDFFLS LSKTLNYLNS 310 320 330 340 350 360 VFTPFVFGLF VFFWIYLRHV VNIRILWSVL TEFRHEGNYV LNFATQQYKC WISLPIVFVL 370 380 390 400 410 IAALQLVNLY WLFLILRILY RLIWQGIQKD ERSDSDSDES AENEESKEKC E

References

[1]

"Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789." Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z., Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X., Jia P., Luedi P., Oefner P.J., David L. , Dietrich F.S., Li Y., Davis R.W., Steinmetz L.M. Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007) [PubMed: 17652520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJM789.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFW02000082 Genomic DNA. Translation: EDN62230.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.24. 984.
Family and domain databases
InterPro	IPR016439. Longevity_assurance_LAG1_LAC1. IPR006634. TLC-dom. IPR013599. TRAM1. [Graphical view]
Pfam	PF08390. TRAM1. 1 hit. PF03798. TRAM_LAG1_CLN8. 1 hit. [Graphical view]
PIRSF	PIRSF005225. LAG1_LAC1. 1 hit.
SMART	SM00724. TLC. 1 hit. [Graphical view]
PROSITE	PS50922. TLC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAG1_YEAS7

Accession

Primary (citable) accession number: A6ZSP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	September 11, 2007
Last modified:	May 31, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program