Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate synthase 1, glyoxysomal

Gene

MLS1

Organism
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This isozyme is necessary for growth on acetate as sole C-source.By similarity

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton acceptorBy similarity
Active sitei457 – 4571Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase 1, glyoxysomal (EC:2.3.3.9)
Gene namesi
Name:MLS1
ORF Names:SCY_4677
OrganismiSaccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Taxonomic identifieri307796 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000007060 Componenti: Unassembled WGS sequence

Subcellular locationi

Glyoxysome By similarity

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Malate synthase 1, glyoxysomalPRO_0000378321Add
BLAST

Proteomic databases

PaxDbiA6ZRW6.

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA6ZRW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi552 – 5543Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiCOG2225.
OrthoDBiEOG7GXPMK.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6ZRW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK
60 70 80 90 100
RKQLLENRQV VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS
110 120 130 140 150
TEITGPPLRN MLINALNAPV NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR
160 170 180 190 200
NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG WHMVEKHLYV DDEPISASIF
210 220 230 240 250
DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF CVAQDYIGIP
260 270 280 290 300
RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN
310 320 330 340 350
DPNHILPNRD QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD
360 370 380 390 400
PAANEKAMTK VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI
410 420 430 440 450
YFIPENVVTA ANLLETKIPN GEITTEGIVQ NLDIGLQYME AWLRGSGCVP
460 470 480 490 500
INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD TGEKVTPELT EKILKEQVER
510 520 530 540 550
LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE IVSTKATPTD

LSKL
Length:554
Mass (Da):62,792
Last modified:September 11, 2007 - v1
Checksum:i6C0698E86D0F1FED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF125227 Genomic DNA. Translation: ABN58639.1.
AAFW02000067 Genomic DNA. Translation: EDN62698.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF125227 Genomic DNA. Translation: ABN58639.1.
AAFW02000067 Genomic DNA. Translation: EDN62698.1.

3D structure databases

ProteinModelPortaliA6ZRW6.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiA6ZRW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG2225.
OrthoDBiEOG7GXPMK.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
    Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
    Genetics 180:1661-1670(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YJM789.

Entry informationi

Entry nameiMASY_YEAS7
AccessioniPrimary (citable) accession number: A6ZRW6
Secondary accession number(s): B0KZS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: September 11, 2007
Last modified: January 7, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.