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A6ZRW6

- MASY_YEAS7

UniProt

A6ZRW6 - MASY_YEAS7

Protein

Malate synthase 1, glyoxysomal

Gene

MLS1

Organism
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    This isozyme is necessary for growth on acetate as sole C-source.By similarity

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei177 – 1771Proton acceptorBy similarity
    Active sitei457 – 4571Proton donorBy similarity

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase 1, glyoxysomal (EC:2.3.3.9)
    Gene namesi
    Name:MLS1
    ORF Names:SCY_4677
    OrganismiSaccharomyces cerevisiae (strain YJM789) (Baker's yeast)
    Taxonomic identifieri307796 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000007060: Unassembled WGS sequence

    Subcellular locationi

    Glyoxysome By similarity

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Malate synthase 1, glyoxysomalPRO_0000378321Add
    BLAST

    Proteomic databases

    PaxDbiA6ZRW6.

    Interactioni

    Subunit structurei

    Homotrimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA6ZRW6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi552 – 5543Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the malate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2225.
    OrthoDBiEOG7GXPMK.

    Family and domain databases

    InterProiIPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001363. Malate_synth. 1 hit.
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6ZRW6-1 [UniParc]FASTAAdd to Basket

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    MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK    50
    RKQLLENRQV VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS 100
    TEITGPPLRN MLINALNAPV NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR 150
    NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG WHMVEKHLYV DDEPISASIF 200
    DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF CVAQDYIGIP 250
    RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN 300
    DPNHILPNRD QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD 350
    PAANEKAMTK VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI 400
    YFIPENVVTA ANLLETKIPN GEITTEGIVQ NLDIGLQYME AWLRGSGCVP 450
    INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD TGEKVTPELT EKILKEQVER 500
    LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE IVSTKATPTD 550
    LSKL 554
    Length:554
    Mass (Da):62,792
    Last modified:September 11, 2007 - v1
    Checksum:i6C0698E86D0F1FED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF125227 Genomic DNA. Translation: ABN58639.1.
    AAFW02000067 Genomic DNA. Translation: EDN62698.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF125227 Genomic DNA. Translation: ABN58639.1 .
    AAFW02000067 Genomic DNA. Translation: EDN62698.1 .

    3D structure databases

    ProteinModelPortali A6ZRW6.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi A6ZRW6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG2225.
    OrthoDBi EOG7GXPMK.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    InterProi IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001363. Malate_synth. 1 hit.
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
      Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
      Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: YJM789.

    Entry informationi

    Entry nameiMASY_YEAS7
    AccessioniPrimary (citable) accession number: A6ZRW6
    Secondary accession number(s): B0KZS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3