A6ZRW3 (TOM70_YEAS7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import receptor subunit TOM70

Alternative name(s):
70 kDa mitochondrial outer membrane protein
Translocase of outer membrane 70 kDa subunit

Gene names

Name:	TOM70
Synonyms:	MAS70, OMP1
ORF Names:	SCY_4674

Organism

Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) [Complete proteome]

Taxonomic identifier

307796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore By similarity.
Subunit structure	Forms part of the TOM (translocase of outer membrane) complex that consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70) By similarity. In the complex interacts with TOM22 By similarity. Interacts with TOM37 By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the Tom70 family. Contains 9 TPR repeats.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Repeat TPR repeat Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Mitochondrial import receptor subunit TOM70		PRO_0000378319
Regions
	Topological domain	1 – 10	10	Mitochondrial intermembrane Potential
	Transmembrane	11 – 30	20	Helical; Potential
	Topological domain	31 – 617	587	Cytoplasmic Potential
	Repeat	99 – 132	34	TPR 1
	Repeat	134 – 165	32	TPR 2
	Repeat	281 – 315	35	TPR 3
	Repeat	363 – 396	34	TPR 4
	Repeat	397 – 430	34	TPR 5
	Repeat	432 – 464	33	TPR 6
	Repeat	465 – 498	34	TPR 7
	Repeat	505 – 541	37	TPR 8
	Repeat	542 – 575	34	TPR 9
Amino acid modifications
	Modified residue	78	1	Phosphoserine By similarity
	Modified residue	174	1	Phosphoserine By similarity
	Modified residue	232	1	Phosphothreonine By similarity
	Modified residue	234	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6ZRW3 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 664B8FC32CCE39A2	FASTA	617	70,123
	10 20 30 40 50 60 MKSFITRNKT AILATVAATG TAIGAYYYYN QLQQQQQRGK KNTINKDEKK DTKDSQKETE 70 80 90 100 110 120 GAKKSTAPSN PPIYPVSSNG EPDFSNKANF TAEEKDKYAL ALKDKGNQFF RNKKYDDAIK 130 140 150 160 170 180 YYNWALELKE DPVFYSNLSA CYVSVGDLKK VVEMSTKALE LKPDYSKVLL RRASANEGLG 190 200 210 220 230 240 KFADAMFDLS VLSLNGDFND ASIEPMLERN LNKQAMSKLK EKFGDIDTAT ATPTELSTQP 250 260 270 280 290 300 AKERKDKQEN LPSVTSMASF FGIFKPELTF ANYDESNEAD KELMNGLSNL YKRSPESYDK 310 320 330 340 350 360 ADESFTKAAR LFEEQLDKNN EDEKLKEKLA ISLEHTGIFK FLKNDPLGAH EDIKKAIELF 370 380 390 400 410 420 PRVNSYIYMA LIMADRNDST EYYNYFDKAL KLDSNNSSVY YHRGQMNFIL QNYDQAGKDF 430 440 450 460 470 480 DKAKELDPEN IFPYIQLACL AYRENKFDDC ETLFSEAKRK FPEAPEVPNF FAEILTDKND 490 500 510 520 530 540 FDKALKQYDL AIELENKLDG IYVGIAPLVG KATLLTRNPT VENFIEATNL LEKASKLDPR 550 560 570 580 590 600 SEQAKIGLAQ MKLQQEDIDE AITLFEESAD LARTMEEKLQ AITFAEAAKV QQRIRSDPVL 610 AKKIQETLAK LREQGLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast." Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M. Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789." Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z., Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X., Jia P., Luedi P., Oefner P.J., David L. , Dietrich F.S., Li Y., Davis R.W., Steinmetz L.M. Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJM789.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF125227 Genomic DNA. Translation: ABN58636.1. AAFW02000067 Genomic DNA. Translation: EDN62695.1.
3D structure databases
ProteinModelPortal	A6ZRW3.
SMR	A6ZRW3. Positions 94-607.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.25.40.10. 3 hits.
InterPro	IPR005687. Tom70. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Pfam	PF00515. TPR_1. 2 hits. [Graphical view]
SMART	SM00028. TPR. 6 hits. [Graphical view]
TIGRFAMs	TIGR00990. 3a0801s09. 1 hit.
PROSITE	PS50005. TPR. 6 hits. PS50293. TPR_REGION. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	979768.

Entry information

Entry name

TOM70_YEAS7

Accession

Primary (citable) accession number: A6ZRW3
Secondary accession number(s): B0KZR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	September 11, 2007
Last modified:	March 6, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program