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Protein

Formate dehydrogenase 1

Gene

FDH1

Organism
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD+-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.UniRule annotation

Catalytic activityi

Formate + NAD+ = CO2 + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97Substrate; via amide nitrogenUniRule annotation1
Binding sitei121SubstrateUniRule annotation1
Binding sitei197NADUniRule annotation1
Binding sitei270NAD; via carbonyl oxygenUniRule annotation1
Sitei272Important for catalytic activityUniRule annotation1
Binding sitei296NADUniRule annotation1
Sitei325Important for catalytic activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi176 – 177NADUniRule annotation2
Nucleotide bindingi244 – 248NADUniRule annotation5
Nucleotide bindingi325 – 328NADUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase 1UniRule annotation (EC:1.2.1.2UniRule annotation)
Short name:
FDHUniRule annotation
Alternative name(s):
NAD-dependent formate dehydrogenaseUniRule annotation
Gene namesi
Name:FDH1
ORF Names:SCY_4914
OrganismiSaccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Taxonomic identifieri307796 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000007060 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003934411 – 376Formate dehydrogenase 1Add BLAST376

Expressioni

Inductioni

Induced by formate.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6ZN46.
SMRiA6ZN46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 121CatalyticUniRule annotationAdd BLAST117
Regioni122 – 326Coenzyme-bindingUniRule annotationAdd BLAST205
Regioni327 – 372CatalyticUniRule annotationAdd BLAST46

Sequence similaritiesi

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.UniRule annotation

Phylogenomic databases

OrthoDBiEOG092C30ZL.

Family and domain databases

CDDicd05302. FDH. 1 hit.
Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase. 1 hit.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR033689. FDH_NAD-dep.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6ZN46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGKVLLVL YEGGKHAEEQ EKLLGCIENE LGIRNFIEEQ GYELVTTIDK
60 70 80 90 100
DPEPTSTVDR ELKDAEIVIT TPFFPAYISR NRIAEAPNLK LCVTAGVGSD
110 120 130 140 150
HVDLEAANER KITVTEVTGS NVVSVAEHVM ATILVLIRNY NGGHQQAING
160 170 180 190 200
EWDIAGVAKN EYDLEDKIIS TVGAGRIGYR VLERLVAFNP KKLLYYDYQE
210 220 230 240 250
LPAEAINRLN EASKLFNGRG DIVQRVEKLE DMVAQSDVVT INCPLHKDSR
260 270 280 290 300
GLFNKKLISH MKDGAYLVNT ARGAICVAED VAEAVKSGKL AGYGGDVWDK
310 320 330 340 350
QPAPKDHPWR TMDNKDHVGN AMTVHISGTS LDAQKRYAQG VKNILNSYFS
360 370
KKFDYRPQDI IVQNGSYATR AYGQKK
Length:376
Mass (Da):41,714
Last modified:September 11, 2007 - v1
Checksum:i67ECDA6F9DDC2A02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFW02000028 Genomic DNA. Translation: EDN64042.1.

Genome annotation databases

EnsemblFungiiEDN64042; EDN64042; SCY_4914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFW02000028 Genomic DNA. Translation: EDN64042.1.

3D structure databases

ProteinModelPortaliA6ZN46.
SMRiA6ZN46.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEDN64042; EDN64042; SCY_4914.

Phylogenomic databases

OrthoDBiEOG092C30ZL.

Family and domain databases

CDDicd05302. FDH. 1 hit.
Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase. 1 hit.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR033689. FDH_NAD-dep.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFDH1_YEAS7
AccessioniPrimary (citable) accession number: A6ZN46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: September 11, 2007
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.