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Protein

Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

SCY_4254

Organism
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (SCY_4254)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.

Protein family/group databases

MEROPSiC44.975.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
ORF Names:SCY_4254
OrganismiSaccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Taxonomic identifieri307796 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000007060 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003776761 – 714Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST714

Proteomic databases

PRIDEiA6ZME2.

Structurei

3D structure databases

ProteinModelPortaliA6ZME2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 321Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST320
Domaini387 – 526SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini559 – 704SIS 2PROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

OrthoDBiEOG092C0ST1.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 2 hits.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6ZME2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGYCNF LIEKTRGEII DTLIEGLQAL EYKEYDSSGI SIQGDELKSL
60 70 80 90 100
NIYKQTGKIS SLKEEIDLYN LNKNLPFISH CGIAHTRRAT HGGLRRANCH
110 120 130 140 150
PHNSDPSNEF VVVHNGVITN FANLKALLVA KGYVFKSDTD TECIPKLYKH
160 170 180 190 200
IYDTSIELGY NLDFHVLTNL VLKELEGSYG LLCTSSHFPD EVVAARKGSP
210 220 230 240 250
LVIGVKGKTD MDVNFVEVEY LDQEEDYLKL NTQTKSSGNV LAAAPVKYNT
260 270 280 290 300
CLRKSPPLRS QYLRNSTTST FNHGSSTETP AENGLPRPME FYLSSDCASL
310 320 330 340 350
ARYVSKVVYL EDNDIAHIYD GELHIHCSKI GSEDFSFRTE LSKIKKGPYD
360 370 380 390 400
NFMQKEIYEQ CETTKNVMRG RVDAFTNRVV LGGLENWLTE LRRAKRIIMI
410 420 430 440 450
ASKASFHSCL AARPIFEELM EVPVNVELAL DFVDRNCCIF RNDVCIFVSR
460 470 480 490 500
SGETTDTINA LNYCIKKEAV TIGVVNCSGS SISRFTHCGV HTNTGPEKGI
510 520 530 540 550
ATTKSYTSQY IALVMIALWM SEDLVSKIER RKEIIQALTI VPSQIKEVLE
560 570 580 590 600
LEPLIIELCD KKLKQHDTFL LLGRGYQFAS ALEGASKMKE ISYVHSESIL
610 620 630 640 650
TNELGHRVLA VASDNPPIIA FATKDAFSPK IASCIDQIIE RKGNPIIICN
660 670 680 690 700
KGHKIWEQDK QKGNVVTLEV PQTVDCLQGI LNVIPLQLIS YWLAIKKDIG
710
VDLPRDSAMS APDI
Length:714
Mass (Da):79,816
Last modified:September 11, 2007 - v1
Checksum:i9E672D9EB22FE108
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFW02000020 Genomic DNA. Translation: EDN64472.1.

Genome annotation databases

EnsemblFungiiEDN64472; EDN64472; SCY_4254.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFW02000020 Genomic DNA. Translation: EDN64472.1.

3D structure databases

ProteinModelPortaliA6ZME2.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC44.975.

Proteomic databases

PRIDEiA6ZME2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEDN64472; EDN64472; SCY_4254.

Phylogenomic databases

OrthoDBiEOG092C0ST1.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 2 hits.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYM084_YEAS7
AccessioniPrimary (citable) accession number: A6ZME2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: September 11, 2007
Last modified: October 5, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.