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Protein

Alpha-defensin 1

Gene

DEFA1

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has broad-spectrum antimicrobial properties (PubMed:17620056, PubMed:19211153). The antimicrobial activity decreases in the present of salt in vitro (PubMed:19211153). Binds anionic phospholipids, which leads to the aggregation of liposomes in vitro (PubMed:25769951). Membrane permeabilization of the target cells is an essential part of the peptide's mode of antimicrobial activity (PubMed:17620056, PubMed:22232283). No hemolytic activity against sheep or horse erythrocytes (PubMed:22232283). Has antibacterial activity against the bacterial horse pathogens Gram-positive R.equi ATCC 33701 P- (minimum bactericidal concentration or MBC=5 µg/ml) and R.equi ATCC 33701 P+ (MBC=5 µg/ml), which are resistant against beta-lactam antibiotics. Also has antibacterial activity against highly infectious wild-type strain R.equi 85F P+ (MBC=5 µg/ml), S.equi subsp. equi (MBC=5 µg/ml), S.equi subsp. zooepidemicus (MBC=5 µg/ml), S.dysgalactiae subsp. equisimilis (MBC=10 µg/ml), S.choleraesuis subsp. choleraesuis serovar Typhimurium (MBC=10 µg/ml), and P.multocida subsp. multocida (MBC=>10 µg/ml) (PubMed:19211153). Probably contributes to the antimicrobial barrier function of the small bowel mucosa (Probable).4 Publications

GO - Molecular functioni

  • phospholipid binding Source: UniProtKB

GO - Biological processi

  • defense response to bacterium Source: UniProtKB

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Defensin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-defensin 11 PublicationImported
Gene namesi
Name:DEFA11 PublicationImported
OrganismiEquus caballus (Horse)Imported
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
PropeptideiPRO_000043858820 – 63By similarityAdd BLAST44
PeptideiPRO_500270544664 – 98Alpha-defensin 1Add BLAST35

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi66 ↔ 96Combined sources1 Publication
Disulfide bondi68 ↔ 84Combined sources1 Publication
Disulfide bondi74 ↔ 95Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PeptideAtlasiA6YB85

Expressioni

Tissue specificityi

Paneth cells of the small bowel.1 Publication

Structurei

Secondary structure

198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi71 – 73Combined sources3
Beta strandi75 – 77Combined sources3
Turni85 – 88Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MXQNMR-A65-98[»]
SMRiA6YB85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.SAAS annotationCurated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG011703

Family and domain databases

InterProiView protein in InterPro
IPR006081 Alpha-defensin
IPR016327 Alpha-defensin_pro
IPR002366 Defensin_propep
PANTHERiPTHR11876 PTHR11876, 1 hit
PfamiView protein in Pfam
PF00323 Defensin_1, 1 hit
PF00879 Defensin_propep, 1 hit
PIRSFiPIRSF001875 Alpha-defensin, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6YB85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLTLLTAL LLLALQVQTQ SLEETADQVP AQDQPGAEAQ DITISFAGDE
60 70 80 90
RSAREASKSL IGTASCTCRR AWICRWGERH SGKCIDQKGS TYRLCCRR
Length:98
Mass (Da):10,843
Last modified:August 21, 2007 - v1
Checksum:iC840F202F5526268
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF379126 mRNA Translation: ABP96800.1
UniGeneiEca.16085

Similar proteinsi

Entry informationi

Entry nameiDEFA1_HORSE
AccessioniPrimary (citable) accession number: A6YB85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2016
Last sequence update: August 21, 2007
Last modified: March 15, 2017
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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