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Protein

Flavonoid 3-O-glucosyltransferase

Gene

UGT78G1

Organism
Medicago truncatula (Barrel medic) (Medicago tribuloides)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the glycosylation of flavonoids at the 3-O-position. Glycosylates the 7-O-position if the 3-O-position is not available. Also able to perform 3-O-glycosylation of anthocyanidins.

Catalytic activityi

UDP-glucose + a flavonoid = UDP + an flavonoid 3-O-D-glucoside.

Kineticsi

  1. KM=89.8 µM for kaempferol1 Publication
  2. KM=28.7 µM for quercitin1 Publication
  3. KM=52.0 µM for pelargonidin1 Publication
  4. KM=71.8 µM for cyanidin1 Publication
  5. KM=1.5 µM for apigenin1 Publication
  6. KM=36.7 µM for genistein1 Publication
  7. KM=1.6 µM for daidzein1 Publication
  8. KM=11.7 µM for biochanin A1 Publication
  9. KM=1.5 µM for formononetin1 Publication
  1. Vmax=1.5830 µmol/min/µg enzyme toward kaempferol1 Publication
  2. Vmax=0.8557 µmol/min/µg enzyme toward quercitin1 Publication
  3. Vmax=0.2025 µmol/min/µg enzyme toward pelargonidin1 Publication
  4. Vmax=0.1698 µmol/min/µg enzyme toward cyanidin1 Publication
  5. Vmax=0.0688 µmol/min/µg enzyme toward apigenin1 Publication
  6. Vmax=1.2050 µmol/min/µg enzyme toward genistein1 Publication
  7. Vmax=0.0530 µmol/min/µg enzyme toward daidzein1 Publication
  8. Vmax=0.3666 µmol/min/µg enzyme toward biochanin A1 Publication
  9. Vmax=0.0879 µmol/min/µg enzyme toward formononetin1 Publication

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Phenylpropanoid metabolism

Enzyme and pathway databases

UniPathwayiUPA00154.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonoid 3-O-glucosyltransferase (EC:2.4.1.-)
Alternative name(s):
UDP glucose:flavonoid 3-O-glucosyltransferase
UDP-glycosyltransferase 78G1
Gene namesi
Name:UGT78G1
Synonyms:GT83F
OrganismiMedicago truncatula (Barrel medic) (Medicago tribuloides)
Taxonomic identifieri3880 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Flavonoid 3-O-glucosyltransferasePRO_0000328919Add
BLAST

Expressioni

Tissue specificityi

Highly expressed in flower buds, flowers and pods. Lower expression in leaves, petioles and stems.1 Publication

Developmental stagei

Expressed in developing seeds, with a maximum at 16 days after pollination. Not expressed in nodules.1 Publication

Inductioni

Strongly down-regulated by wounding or by methyl jasmonate.1 Publication

Gene expression databases

ExpressionAtlasiA6XNC6. differential.

Structurei

Secondary structure

1
454
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195Combined sources
Beta strandi23 – 264Combined sources
Helixi27 – 4014Combined sources
Beta strandi44 – 507Combined sources
Helixi52 – 576Combined sources
Beta strandi60 – 634Combined sources
Beta strandi69 – 735Combined sources
Helixi90 – 11425Combined sources
Beta strandi120 – 1245Combined sources
Helixi130 – 1367Combined sources
Beta strandi140 – 1456Combined sources
Helixi149 – 1568Combined sources
Helixi158 – 1636Combined sources
Helixi167 – 1704Combined sources
Helixi187 – 1893Combined sources
Beta strandi194 – 1963Combined sources
Helixi201 – 21212Combined sources
Helixi213 – 2153Combined sources
Beta strandi219 – 2235Combined sources
Helixi225 – 2273Combined sources
Helixi229 – 2368Combined sources
Beta strandi242 – 2443Combined sources
Helixi248 – 2514Combined sources
Helixi263 – 2686Combined sources
Beta strandi275 – 2795Combined sources
Turni281 – 2833Combined sources
Helixi288 – 30114Combined sources
Beta strandi305 – 3084Combined sources
Helixi313 – 3164Combined sources
Helixi321 – 3244Combined sources
Turni325 – 3284Combined sources
Beta strandi329 – 3346Combined sources
Helixi337 – 3426Combined sources
Beta strandi346 – 3516Combined sources
Helixi355 – 36410Combined sources
Beta strandi368 – 3703Combined sources
Helixi377 – 3859Combined sources
Beta strandi390 – 3934Combined sources
Helixi395 – 3973Combined sources
Helixi401 – 41212Combined sources
Helixi415 – 43420Combined sources
Helixi440 – 45213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HBFX-ray2.10A1-454[»]
3HBJX-ray2.10A1-454[»]
ProteinModelPortaliA6XNC6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA6XNC6.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 544Poly-Thr

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

OMAiLWTAGPR.
OrthoDBiEOG09360BMW.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6XNC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTFKNEMNG NNLLHVAVLA FPFGTHAAPL LSLVKKIATE APKVTFSFFC
60 70 80 90 100
TTTTNDTLFS RSNEFLPNIK YYNVHDGLPK GYVSSGNPRE PIFLFIKAMQ
110 120 130 140 150
ENFKHVIDEA VAETGKNITC LVTDAFFWFG ADLAEEMHAK WVPLWTAGPH
160 170 180 190 200
SLLTHVYTDL IREKTGSKEV HDVKSIDVLP GFPELKASDL PEGVIKDIDV
210 220 230 240 250
PFATMLHKMG LELPRANAVA INSFATIHPL IENELNSKFK LLLNVGPFNL
260 270 280 290 300
TTPQRKVSDE HGCLEWLDQH ENSSVVYISF GSVVTPPPHE LTALAESLEE
310 320 330 340 350
CGFPFIWSFR GDPKEKLPKG FLERTKTKGK IVAWAPQVEI LKHSSVGVFL
360 370 380 390 400
THSGWNSVLE CIVGGVPMIS RPFFGDQGLN TILTESVLEI GVGVDNGVLT
410 420 430 440 450
KESIKKALEL TMSSEKGGIM RQKIVKLKES AFKAVEQNGT SAMDFTTLIQ

IVTS
Length:454
Mass (Da):50,158
Last modified:August 21, 2007 - v1
Checksum:i5026E098D8F2EAE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ875464 mRNA. Translation: ABI94025.1.
RefSeqiXP_003610163.1. XM_003610115.2.
UniGeneiMtr.19386.

Genome annotation databases

EnsemblPlantsiAES92360; AES92360; MTR_4g128690.
GeneIDi11445564.
GrameneiAES92360; AES92360; MTR_4g128690.
KEGGimtr:MTR_4g128690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ875464 mRNA. Translation: ABI94025.1.
RefSeqiXP_003610163.1. XM_003610115.2.
UniGeneiMtr.19386.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HBFX-ray2.10A1-454[»]
3HBJX-ray2.10A1-454[»]
ProteinModelPortaliA6XNC6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAES92360; AES92360; MTR_4g128690.
GeneIDi11445564.
GrameneiAES92360; AES92360; MTR_4g128690.
KEGGimtr:MTR_4g128690.

Phylogenomic databases

OMAiLWTAGPR.
OrthoDBiEOG09360BMW.

Enzyme and pathway databases

UniPathwayiUPA00154.

Miscellaneous databases

EvolutionaryTraceiA6XNC6.

Gene expression databases

ExpressionAtlasiA6XNC6. differential.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUGFGT_MEDTR
AccessioniPrimary (citable) accession number: A6XNC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

For the flavonols kaempferol and quercetin, a significant activity was also observed with UDP-galactose as sugar donor. The reverse reaction (deglycosylation) is observed, but not with formononetin 7-O-glucoside.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.