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Protein

Flavonoid 3-O-glucosyltransferase

Gene

UGT78G1

Organism
Medicago truncatula (Barrel medic) (Medicago tribuloides)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the glycosylation of flavonoids at the 3-O-position. Glycosylates the 7-O-position if the 3-O-position is not available. Also able to perform 3-O-glycosylation of anthocyanidins.

Catalytic activityi

UDP-glucose + a flavonoid = UDP + an flavonoid 3-O-D-glucoside.

Kineticsi

  1. KM=89.8 µM for kaempferol1 Publication
  2. KM=28.7 µM for quercitin1 Publication
  3. KM=52.0 µM for pelargonidin1 Publication
  4. KM=71.8 µM for cyanidin1 Publication
  5. KM=1.5 µM for apigenin1 Publication
  6. KM=36.7 µM for genistein1 Publication
  7. KM=1.6 µM for daidzein1 Publication
  8. KM=11.7 µM for biochanin A1 Publication
  9. KM=1.5 µM for formononetin1 Publication
  1. Vmax=1.5830 µmol/min/µg enzyme toward kaempferol1 Publication
  2. Vmax=0.8557 µmol/min/µg enzyme toward quercitin1 Publication
  3. Vmax=0.2025 µmol/min/µg enzyme toward pelargonidin1 Publication
  4. Vmax=0.1698 µmol/min/µg enzyme toward cyanidin1 Publication
  5. Vmax=0.0688 µmol/min/µg enzyme toward apigenin1 Publication
  6. Vmax=1.2050 µmol/min/µg enzyme toward genistein1 Publication
  7. Vmax=0.0530 µmol/min/µg enzyme toward daidzein1 Publication
  8. Vmax=0.3666 µmol/min/µg enzyme toward biochanin A1 Publication
  9. Vmax=0.0879 µmol/min/µg enzyme toward formononetin1 Publication

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Phenylpropanoid metabolism

Enzyme and pathway databases

UniPathwayiUPA00154.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonoid 3-O-glucosyltransferase (EC:2.4.1.-)
Alternative name(s):
UDP glucose:flavonoid 3-O-glucosyltransferase
UDP-glycosyltransferase 78G1
Gene namesi
Name:UGT78G1
Synonyms:GT83F
OrganismiMedicago truncatula (Barrel medic) (Medicago tribuloides)
Taxonomic identifieri3880 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289191 – 454Flavonoid 3-O-glucosyltransferaseAdd BLAST454

Expressioni

Tissue specificityi

Highly expressed in flower buds, flowers and pods. Lower expression in leaves, petioles and stems.1 Publication

Developmental stagei

Expressed in developing seeds, with a maximum at 16 days after pollination. Not expressed in nodules.1 Publication

Inductioni

Strongly down-regulated by wounding or by methyl jasmonate.1 Publication

Gene expression databases

ExpressionAtlasiA6XNC6. baseline.

Structurei

Secondary structure

1454
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 19Combined sources5
Beta strandi23 – 26Combined sources4
Helixi27 – 40Combined sources14
Beta strandi44 – 50Combined sources7
Helixi52 – 57Combined sources6
Beta strandi60 – 63Combined sources4
Beta strandi69 – 73Combined sources5
Helixi90 – 114Combined sources25
Beta strandi120 – 124Combined sources5
Helixi130 – 136Combined sources7
Beta strandi140 – 145Combined sources6
Helixi149 – 156Combined sources8
Helixi158 – 163Combined sources6
Helixi167 – 170Combined sources4
Helixi187 – 189Combined sources3
Beta strandi194 – 196Combined sources3
Helixi201 – 212Combined sources12
Helixi213 – 215Combined sources3
Beta strandi219 – 223Combined sources5
Helixi225 – 227Combined sources3
Helixi229 – 236Combined sources8
Beta strandi242 – 244Combined sources3
Helixi248 – 251Combined sources4
Helixi263 – 268Combined sources6
Beta strandi275 – 279Combined sources5
Turni281 – 283Combined sources3
Helixi288 – 301Combined sources14
Beta strandi305 – 308Combined sources4
Helixi313 – 316Combined sources4
Helixi321 – 324Combined sources4
Turni325 – 328Combined sources4
Beta strandi329 – 334Combined sources6
Helixi337 – 342Combined sources6
Beta strandi346 – 351Combined sources6
Helixi355 – 364Combined sources10
Beta strandi368 – 370Combined sources3
Helixi377 – 385Combined sources9
Beta strandi390 – 393Combined sources4
Helixi395 – 397Combined sources3
Helixi401 – 412Combined sources12
Helixi415 – 434Combined sources20
Helixi440 – 452Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HBFX-ray2.10A1-454[»]
3HBJX-ray2.10A1-454[»]
ProteinModelPortaliA6XNC6.
SMRiA6XNC6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA6XNC6.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 54Poly-Thr4

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

OMAiLWTAGPR.
OrthoDBiEOG09360BMW.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6XNC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTFKNEMNG NNLLHVAVLA FPFGTHAAPL LSLVKKIATE APKVTFSFFC
60 70 80 90 100
TTTTNDTLFS RSNEFLPNIK YYNVHDGLPK GYVSSGNPRE PIFLFIKAMQ
110 120 130 140 150
ENFKHVIDEA VAETGKNITC LVTDAFFWFG ADLAEEMHAK WVPLWTAGPH
160 170 180 190 200
SLLTHVYTDL IREKTGSKEV HDVKSIDVLP GFPELKASDL PEGVIKDIDV
210 220 230 240 250
PFATMLHKMG LELPRANAVA INSFATIHPL IENELNSKFK LLLNVGPFNL
260 270 280 290 300
TTPQRKVSDE HGCLEWLDQH ENSSVVYISF GSVVTPPPHE LTALAESLEE
310 320 330 340 350
CGFPFIWSFR GDPKEKLPKG FLERTKTKGK IVAWAPQVEI LKHSSVGVFL
360 370 380 390 400
THSGWNSVLE CIVGGVPMIS RPFFGDQGLN TILTESVLEI GVGVDNGVLT
410 420 430 440 450
KESIKKALEL TMSSEKGGIM RQKIVKLKES AFKAVEQNGT SAMDFTTLIQ

IVTS
Length:454
Mass (Da):50,158
Last modified:August 21, 2007 - v1
Checksum:i5026E098D8F2EAE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ875464 mRNA. Translation: ABI94025.1.
RefSeqiXP_003610163.1. XM_003610115.2.
UniGeneiMtr.19386.

Genome annotation databases

EnsemblPlantsiAES92360; AES92360; MTR_4g128690.
GeneIDi11445564.
GrameneiAES92360; AES92360; MTR_4g128690.
KEGGimtr:MTR_4g128690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ875464 mRNA. Translation: ABI94025.1.
RefSeqiXP_003610163.1. XM_003610115.2.
UniGeneiMtr.19386.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HBFX-ray2.10A1-454[»]
3HBJX-ray2.10A1-454[»]
ProteinModelPortaliA6XNC6.
SMRiA6XNC6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAES92360; AES92360; MTR_4g128690.
GeneIDi11445564.
GrameneiAES92360; AES92360; MTR_4g128690.
KEGGimtr:MTR_4g128690.

Phylogenomic databases

OMAiLWTAGPR.
OrthoDBiEOG09360BMW.

Enzyme and pathway databases

UniPathwayiUPA00154.

Miscellaneous databases

EvolutionaryTraceiA6XNC6.

Gene expression databases

ExpressionAtlasiA6XNC6. baseline.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUGFGT_MEDTR
AccessioniPrimary (citable) accession number: A6XNC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: August 21, 2007
Last modified: November 30, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

For the flavonols kaempferol and quercetin, a significant activity was also observed with UDP-galactose as sugar donor. The reverse reaction (deglycosylation) is observed, but not with formononetin 7-O-glucoside.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.