Reviewed, UniProtKB/Swiss-Prot A6X798 (3HAO_OCHA4)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Ordered Locus Names:

Oant_4402

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O(2) = 2-amino-3-carboxymuconate semialdehyde.
Cofactor	Binds 2 Fe(2+) ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	3-hydroxyanthranilate 3,4-dioxygenase		PRO_0000309576
Sites
	Metal binding	50	1	Iron 1; catalytic By similarity
	Metal binding	56	1	Iron 1; catalytic By similarity
	Metal binding	96	1	Iron 1; catalytic By similarity
	Metal binding	126	1	Iron 2 By similarity
	Metal binding	129	1	Iron 2 By similarity
	Metal binding	163	1	Iron 2 By similarity
	Metal binding	166	1	Iron 2 By similarity
	Binding site	46	1	Dioxygen By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6X798-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 2E0B4D684E611342	FASTA	182	20,693
	10 20 30 40 50 60 MTKLSAFNFQ KWIDEHKHLL KPPVGNQLVF KDADLMVTVV GGPNKRTDYH DDPVEEFFYQ 70 80 90 100 110 120 LKGDMLLKLH DTSTGEFYDV PIREGDIFLL PAHIRHSPQR PQEGSIGLVI EPARPEGVLD 130 140 150 160 170 180 AVEWYCFECS GLVHRAEVDL ESIVDDLPPL YAAFYNDEKL RTCPHCNTVH PGKNPPEGWV AL

References

[1]

"Complete sequence of chromosome 2 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000759 Genomic DNA. Translation: ABS17102.1.
RefSeq	YP_001372931.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5382663.
GenomeReviews	Gene locus Oant_4402 in contig CP000759_GR.
KEGG	oan:Oant_4402.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00825. [Tree]
InterPro	IPR010329. 3hydroanth_dOase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_OCHA4

Accession

Primary (citable) accession number: A6X798

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	August 21, 2007
Last modified:	November 4, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)