Reviewed, UniProtKB/Swiss-Prot A6X792 (ASPD_OCHA4)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Oant_4396

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067309
Sites
	Active site	221	1	By similarity
	Binding site	125	1	NAD; via amide nitrogen By similarity
	Binding site	191	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6X792-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 32BA43AEF0CCFC12	FASTA	268	27,854
	10 20 30 40 50 60 MSVSETIVLV GWGAIGKRVA DLLAERKSSV RIGAVAVRDR SASRDRLPAG AVLIENPAEL 70 80 90 100 110 120 AASGASLVVE AAGRPSVLPW GEAALSTGMD FAVSSTSAFV DDALFQRLKD AAAASGAKLI 130 140 150 160 170 180 IPPGALGGID ALSAASRLSI ESVEHRIIKP AKAWAGTQAA QLVPLDEISE ATVFFTDTAR 190 200 210 220 230 240 KAADAFPQNA NVAVITSLAG IGLDRTRVTL VADPAARLNT HEIIAEGDFG RMHLRFENGP 250 260 LATNPKSSEM TALNLVRAIE NRVATTVI

References

[1]

"Complete sequence of chromosome 2 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000759 Genomic DNA. Translation: ABS17096.1.
RefSeq	YP_001372925.1.
3D structure databases
SMR	A6X792. Positions 7-268.
ModBase	Search...
Protein-protein interaction databases
STRING	A6X792.
Genome annotation databases
GeneID	5381939.
GenomeReviews	Gene locus Oant_4396 in contig CP000759_GR.
KEGG	oan:Oant_4396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1712.
HOGENOM	HBG649642.
OMA	ECAGHSA.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_OCHA4

Accession

Primary (citable) accession number: A6X792

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	January 19, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)