A6X3F1 (LPXK_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Oant_3047

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000049901
Regions
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A6X3F1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 05F57C69F008A714	FASTA	341	36,506
	10 20 30 40 50 60 MVSEAPPFWW DKPDWRALSL APAAWLYGAV AGRRLLKAEP PKISLPVLCV GNFTVGGAGK 70 80 90 100 110 120 TPTAIAFSAG AKARGLNPGI VSRGYGGAYK GLHVVDPAHD SARYVGDEPL LLARHASVAL 130 140 150 160 170 180 CPDRLKAARH LQALGCDFII MDDGFQSARL FADFSLLVVD AARGIGNGKV IPAGPLRAPL 190 200 210 220 230 240 TDQMRKTDAL LRIGKGDGAD FVIRQTARAG RPIYEARLKP SSTTPIAGNR WLAFAGIGNP 250 260 270 280 290 300 EKFYASVAEA GGEIVETCSF PDHHSFASDD IRNLTDTARR QGLGLITTAK DQVRLATMIG 310 320 330 340 VPAGFLTKLA VLNVDLKFDR NDALDHILDT VIERFKSRVT S

References

[1]

"Complete sequence of chromosome 2 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000759 Genomic DNA. Translation: ABS15755.1.
RefSeq	YP_001371584.1. NC_009668.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6X3F1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5381358.
GenomeReviews	Gene locus Oant_3047 in contig CP000759_GR.
KEGG	oan:Oant_3047.
PATRIC	20471438. VBIOchAnt73124_3198.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	OANT439375:OANT_3047-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_OCHA4

Accession

Primary (citable) accession number: A6X3F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program