A6X2S8 (BIOB_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	Oant_2823

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum › Ochrobactrum anthropi

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381512
Sites
	Metal binding	70	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	74	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	77	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	114	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	145	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	205	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	277	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6X2S8 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D9FE099764220AB3	FASTA	336	36,718
	10 20 30 40 50 60 MLNMVQSNPK GVEKTASKSE QDAWTLASAR LLYDLPFNDL LFEAQNVHRA NFDPNRVQLS 70 80 90 100 110 120 KLLNIKTGGC PEDCGYCSQS AHHASGLKAS KLMSLDTVLE EAQKAKDSGA TRYCMGAAWR 130 140 150 160 170 180 SPKPRDEPAI AEMVKQVKAL GLETCMTLGM LSPDQAQTFA EAGLDYYNHN IDTSERFYPQ 190 200 210 220 230 240 VITTRSFDDR LETLAHVREA GIKVCSGGIL GLGETEDDRI DMLVTLANLP TPPESVPINM 250 260 270 280 290 300 LIPMPGSRLE KASPVDPIAF VRIIALARLM MPQSHVRLTA GRNSMSDEMQ ALCFFAGANS 310 320 330 IFIGDTLLTA ANPGEDRDTS LMRRLGLTAD TLDNHA

References

[1]

"Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts." Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E. J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000759 Genomic DNA. Translation: ABS15532.1.
RefSeq	YP_001371361.1. NC_009668.1.
3D structure databases
ProteinModelPortal	A6X2S8.
SMR	A6X2S8. Positions 24-327.
ModBase	Search...
Protein-protein interaction databases
STRING	439375.Oant_2823.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS15532; ABS15532; Oant_2823.
GeneID	5382596.
KEGG	oan:Oant_2823.
PATRIC	20470968. VBIOchAnt73124_2965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
OMA	RIMMPAS.
ProtClustDB	CLSK2303100.
Enzyme and pathway databases
BioCyc	OANT439375:GJIT-2874-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_OCHA4

Accession

Primary (citable) accession number: A6X2S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program