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A6X2S8

- BIOB_OCHA4

UniProt

A6X2S8 - BIOB_OCHA4

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Protein
Biotin synthase
Gene
bioB, Oant_2823
Organism
Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi145 – 1451Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi205 – 2051Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi277 – 2771Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciOANT439375:GJIT-2874-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Oant_2823
OrganismiOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
Taxonomic identifieri439375 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum
ProteomesiUP000002301: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Biotin synthaseUniRule annotation
PRO_0000381512Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi439375.Oant_2823.

Structurei

3D structure databases

ProteinModelPortaliA6X2S8.
SMRiA6X2S8. Positions 24-327.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6X2S8-1 [UniParc]FASTAAdd to Basket

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MLNMVQSNPK GVEKTASKSE QDAWTLASAR LLYDLPFNDL LFEAQNVHRA    50
NFDPNRVQLS KLLNIKTGGC PEDCGYCSQS AHHASGLKAS KLMSLDTVLE 100
EAQKAKDSGA TRYCMGAAWR SPKPRDEPAI AEMVKQVKAL GLETCMTLGM 150
LSPDQAQTFA EAGLDYYNHN IDTSERFYPQ VITTRSFDDR LETLAHVREA 200
GIKVCSGGIL GLGETEDDRI DMLVTLANLP TPPESVPINM LIPMPGSRLE 250
KASPVDPIAF VRIIALARLM MPQSHVRLTA GRNSMSDEMQ ALCFFAGANS 300
IFIGDTLLTA ANPGEDRDTS LMRRLGLTAD TLDNHA 336
Length:336
Mass (Da):36,718
Last modified:August 21, 2007 - v1
Checksum:iD9FE099764220AB3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000759 Genomic DNA. Translation: ABS15532.1.
RefSeqiYP_001371361.1. NC_009668.1.

Genome annotation databases

EnsemblBacteriaiABS15532; ABS15532; Oant_2823.
GeneIDi5382596.
KEGGioan:Oant_2823.
PATRICi20470968. VBIOchAnt73124_2965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000759 Genomic DNA. Translation: ABS15532.1 .
RefSeqi YP_001371361.1. NC_009668.1.

3D structure databases

ProteinModelPortali A6X2S8.
SMRi A6X2S8. Positions 24-327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 439375.Oant_2823.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS15532 ; ABS15532 ; Oant_2823 .
GeneIDi 5382596.
KEGGi oan:Oant_2823.
PATRICi 20470968. VBIOchAnt73124_2965.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci OANT439375:GJIT-2874-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts."
    Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E.
    J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Entry informationi

Entry nameiBIOB_OCHA4
AccessioniPrimary (citable) accession number: A6X2S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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