A6X0K5 (SYE1_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Oant_2043

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367722
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6X0K5 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: C9A27760AD05C689	FASTA	473	52,210
	10 20 30 40 50 60 MSKPVVTRFA PSPTGYLHIG GARTALFNWL YAKHSGGKML LRIEDTDRER STDAATAAIL 70 80 90 100 110 120 DGLTWLGLDW DGDAISQFER APRHREVAEE LVALGKAYYC YATPEELAEM REKAREEGRP 130 140 150 160 170 180 PRYDGRWRDR DPSEAPAGVK PVIRIKAPQE GETLVQDAVQ GDVRFPNKDL DDFIILRSDG 190 200 210 220 230 240 TPTYMHAVVV DDHDMGVTHI IRGDDHLTNA ARQTVIYDAM GWDVPQMSHI PLIHGADGAK 250 260 270 280 290 300 LSKRHGALGV DAYRAMGYLP AALRNYLVRL GWSHGDDEIM STEQMIEWFD VKDINKGAAR 310 320 330 340 350 360 FDFQKLEAIN GVYMRASDDK ALYDALIAVL PEIEGGKEIA DALDDKRSTQ LLAAMPGLKD 370 380 390 400 410 420 RAKTLVELAD SSKFIFAARP LDLDEKAASL LNDEGRAVLK SVLPDLEAAG EWTVESLDGV 430 440 450 460 470 VRTHAEKTGL KLGKIAQPLR ASLTGRATSP GVFDVLFVLG REESLGRIRD QIG

References

[1]

"Complete sequence of chromosome 1 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000758 Genomic DNA. Translation: ABS14759.1.
RefSeq	YP_001370588.1. NC_009667.1.
3D structure databases
ProteinModelPortal	A6X0K5.
ModBase	Search...
Protein-protein interaction databases
STRING	A6X0K5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5380045.
GenomeReviews	Gene locus Oant_2043 in contig CP000758_GR.
KEGG	oan:Oant_2043.
PATRIC	20469303. VBIOchAnt73124_2148.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	OANT439375:OANT_2043-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_OCHA4

Accession

Primary (citable) accession number: A6X0K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program