A6WZC8 (HEM6_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	Oant_1616

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000119808
Regions
	Region	73 – 82	10	Important for dimerization By similarity
	Region	133 – 135	3	Substrate binding By similarity
	Region	269 – 304	36	Important for dimerization By similarity
	Region	287 – 292	6	Substrate binding By similarity
Sites
	Active site	131	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	200	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6WZC8 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: EB68A01CF397C140	FASTA	304	35,152
	10 20 30 40 50 60 MQRDEIPAII PTDIEEKKQA AQRWFEELRD RICASYEQLE DELQGPLSDR EPGRFVRTPW 70 80 90 100 110 120 QKDEGRGGGG VMSIMHGRVF EKVGVHVSTV YGEFSPEFRK QIPGAEEDPR YWASGISLIA 130 140 150 160 170 180 HPQNPNVPAV HMNTRMVVTT RQWFGGGADL TPVLDNRRTQ DDPDTLAFHK AFRFICEKHK 190 200 210 220 230 240 DIVDYQRVKD WCDEYFFLPH RNEARGTGGI FYDWLHSPDE KGGWDADFAY TRDVGRGFSV 250 260 270 280 290 300 VYPHLVRQNF NKDWTDADRN EQLIRRGRYV EFNLLYDRGT IFGLKTGGNV NSILSSMPPV VKWP

References

[1]

"Complete sequence of chromosome 1 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000758 Genomic DNA. Translation: ABS14332.1.
RefSeq	YP_001370161.1. NC_009667.1.
3D structure databases
ProteinModelPortal	A6WZC8.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WZC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5378562.
GenomeReviews	Gene locus Oant_1616 in contig CP000758_GR.
KEGG	oan:Oant_1616.
PATRIC	20468399. VBIOchAnt73124_1703.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	VKAYLLD.
ProtClustDB	PRK05330.
Enzyme and pathway databases
BioCyc	OANT439375:OANT_1616-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_OCHA4

Accession

Primary (citable) accession number: A6WZC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program