A6WYD2 (MASZ_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Oant_1269

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Malate synthase G HAMAP MF_00641		PRO_1000056916
Sites
	Active site	345	1	Proton acceptor By similarity
	Active site	636	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6WYD2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 89C758B66592CB10	FASTA	728	79,664
	10 20 30 40 50 60 MVSQKAGNYV EIEGLRVAPE LVEFLAKEAA PGTGVEPEKF WKGFAAIIRD LAPKNRALLA 70 80 90 100 110 120 KRDDLQAKID AWYKQNRDKG YSQADYQQFL KNIGYLLPEG GEFSVSTTNV DPEITHIAGP 130 140 150 160 170 180 QLVVPVMNAR YALNAANARW GSLYDALYGT DAISDADGGE KGKGYNPKRG EKVIAWAKNF 190 200 210 220 230 240 LDESAPLATG NWANVAGLAV KDGKLEIKLT DGSATALKDA NQFKGYNGDA AAPTNVLLAK 250 260 270 280 290 300 NNMHVDIVVN ADHPIGKTDP AHIADVVLES AVSTIQDCED SIAAVDAEDK VAVYRNWLGL 310 320 330 340 350 360 MNGKLEDTFE KNGKQMTRRL NGDRSYKAAD GSTLSLKGRS LMLVRNVGHL MTNPAIIDAD 370 380 390 400 410 420 GHEVPEGIMD AAFTSLIALH DIGPNGRHMN SREGSVYIVK PKMHGPEEVA FANEIFTRTE 430 440 450 460 470 480 EMLGMKPNTL KIGIMDEERR TTVNLKEAIR AAKERVVFIN TGFLDRTGDE IHTSMEAGPM 490 500 510 520 530 540 IRKGDMKQAA WIGAYEQWNV DIGLECGLSG HAQIGKGMWA MPDLMAAMLE QKIAHPKAGA 550 560 570 580 590 600 NTAWVPSPTA ATLHATHYHQ VDVAAVQAKL KSRPRAKLDD ILSVPVATRP NWTPEDIQHE 610 620 630 640 650 660 IDNNAQGILG YVVRWVDQGV GCSKVPDINN VGLMEDRATL RISAQHIANW LYHGVVSEAQ 670 680 690 700 710 720 VMETMKRMAA VVDKQNEGDA LYRPMAADFD KSIAFQAACD LVFKGREQPN GYTEPVLHRR RLELKAQG

References

[1]

"Complete sequence of chromosome 1 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000758 Genomic DNA. Translation: ABS13986.1.
RefSeq	YP_001369815.1. NC_009667.1.
3D structure databases
ProteinModelPortal	A6WYD2.
SMR	A6WYD2. Positions 10-728.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WYD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5378625.
GenomeReviews	Gene locus Oant_1269 in contig CP000758_GR.
KEGG	oan:Oant_1269.
PATRIC	20467633. VBIOchAnt73124_1330.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HBG350005.
OMA	PKMHGPD.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	OANT439375:OANT_1269-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_OCHA4

Accession

Primary (citable) accession number: A6WYD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program