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A6WYD2

- MASZ_OCHA4

UniProt

A6WYD2 - MASZ_OCHA4

Protein

Malate synthase G

Gene

glcB

Organism
Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei123 – 1231Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei281 – 2811Acetyl-CoAUniRule annotation
    Binding sitei318 – 3181Acetyl-CoAUniRule annotation
    Active sitei345 – 3451Proton acceptorUniRule annotation
    Binding sitei345 – 3451GlyoxylateUniRule annotation
    Metal bindingi437 – 4371MagnesiumUniRule annotation
    Binding sitei437 – 4371GlyoxylateUniRule annotation
    Metal bindingi465 – 4651MagnesiumUniRule annotation
    Binding sitei546 – 5461Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei636 – 6361Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciOANT439375:GJIT-1286-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Oant_1269
    OrganismiOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
    Taxonomic identifieri439375 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum
    ProteomesiUP000002301: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 728728Malate synthase GPRO_1000056916Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei622 – 6221Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi439375.Oant_1269.

    Structurei

    3D structure databases

    ProteinModelPortaliA6WYD2.
    SMRiA6WYD2. Positions 10-728.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni130 – 1312Acetyl-CoA bindingUniRule annotation
    Regioni462 – 4654Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6WYD2-1 [UniParc]FASTAAdd to Basket

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    MVSQKAGNYV EIEGLRVAPE LVEFLAKEAA PGTGVEPEKF WKGFAAIIRD    50
    LAPKNRALLA KRDDLQAKID AWYKQNRDKG YSQADYQQFL KNIGYLLPEG 100
    GEFSVSTTNV DPEITHIAGP QLVVPVMNAR YALNAANARW GSLYDALYGT 150
    DAISDADGGE KGKGYNPKRG EKVIAWAKNF LDESAPLATG NWANVAGLAV 200
    KDGKLEIKLT DGSATALKDA NQFKGYNGDA AAPTNVLLAK NNMHVDIVVN 250
    ADHPIGKTDP AHIADVVLES AVSTIQDCED SIAAVDAEDK VAVYRNWLGL 300
    MNGKLEDTFE KNGKQMTRRL NGDRSYKAAD GSTLSLKGRS LMLVRNVGHL 350
    MTNPAIIDAD GHEVPEGIMD AAFTSLIALH DIGPNGRHMN SREGSVYIVK 400
    PKMHGPEEVA FANEIFTRTE EMLGMKPNTL KIGIMDEERR TTVNLKEAIR 450
    AAKERVVFIN TGFLDRTGDE IHTSMEAGPM IRKGDMKQAA WIGAYEQWNV 500
    DIGLECGLSG HAQIGKGMWA MPDLMAAMLE QKIAHPKAGA NTAWVPSPTA 550
    ATLHATHYHQ VDVAAVQAKL KSRPRAKLDD ILSVPVATRP NWTPEDIQHE 600
    IDNNAQGILG YVVRWVDQGV GCSKVPDINN VGLMEDRATL RISAQHIANW 650
    LYHGVVSEAQ VMETMKRMAA VVDKQNEGDA LYRPMAADFD KSIAFQAACD 700
    LVFKGREQPN GYTEPVLHRR RLELKAQG 728
    Length:728
    Mass (Da):79,664
    Last modified:August 21, 2007 - v1
    Checksum:i89C758B66592CB10
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000758 Genomic DNA. Translation: ABS13986.1.
    RefSeqiYP_001369815.1. NC_009667.1.

    Genome annotation databases

    EnsemblBacteriaiABS13986; ABS13986; Oant_1269.
    GeneIDi5378625.
    KEGGioan:Oant_1269.
    PATRICi20467633. VBIOchAnt73124_1330.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000758 Genomic DNA. Translation: ABS13986.1 .
    RefSeqi YP_001369815.1. NC_009667.1.

    3D structure databases

    ProteinModelPortali A6WYD2.
    SMRi A6WYD2. Positions 10-728.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 439375.Oant_1269.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS13986 ; ABS13986 ; Oant_1269 .
    GeneIDi 5378625.
    KEGGi oan:Oant_1269.
    PATRICi 20467633. VBIOchAnt73124_1330.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci OANT439375:GJIT-1286-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts."
      Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E.
      J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

    Entry informationi

    Entry nameiMASZ_OCHA4
    AccessioniPrimary (citable) accession number: A6WYD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3