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A6WXW1 (SYL_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Oant_1095
OrganismOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]
Taxonomic identifier439375 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum

Protein attributes

Sequence length877 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 877877Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009384

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif628 – 6325"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6WXW1 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 29A56EA8E314F5F4

FASTA87798,210
        10         20         30         40         50         60 
MAAERYNPRV AEAHWQKVWE EDRTFETDNS DSREKYYVLE MFPYPSGRIH MGHVRNYAMG 

        70         80         90        100        110        120 
DVVARYKRAK GFNVLHPMGW DAFGMPAENA AMQNKVHPKE WTYQNIATMR SQLKSMGLSL 

       130        140        150        160        170        180 
DWAREFATCD VEYYHRQQML FIDLFEKGLV TRKTSKVNWD PVDNTVLANE QVVDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFKITDFS EELLAGLDTL DQWPEKVRLM QRNWIGKSEG LQVRFTLDGK 

       250        260        270        280        290        300 
TAPEGFSEVE VYTTRPDTLF GAAFVGISAD HPLAKKLAEN NASLTGFIEE CHQHGTSLAA 

       310        320        330        340        350        360 
LETAEKKGFD TGVKVKHPFD ENWELPVYVA NFVLMEYGTG AVFGCPAHDQ RDLDFANKYK 

       370        380        390        400        410        420 
LKVTPVVMPK GEDAAGFSIG ETAYTDDGVM INSRFLDGMT PEAAFNEVAN RLEQTSLGNS 

       430        440        450        460        470        480 
PQASRKVQFR LRDWGISRQR YWGCPIPMIH CESCGVNPVP RADLPVKLPD DVEFDRPGNP 

       490        500        510        520        530        540 
LDRHATWRHV KCPKCGADAR RETDTMDTFV DSSWYYARFT APWENEPTDR KVADHWLPVD 

       550        560        570        580        590        600 
QYIGGIEHAI LHLLYSRFFT RAMKVAGHVG IDEPFKGLFT QGMVVHETYK ANGQWVAPAD 

       610        620        630        640        650        660 
IRIEETDGKR SAALLSTGEP VEIGSIEKMS KSKKNVVDPD DIIASYGADT ARWFMLSDSP 

       670        680        690        700        710        720 
PERDVIWTEA GAEGAHRFVQ RVWRLVAEAA EHLKDVAPKT GTQGEALVVS KAAHKAVKAV 

       730        740        750        760        770        780 
GDDIEKLAFN RGVARLYELV NTAAASLQQV ASGNADDELK SAVRDVTEKL ILMLAPMMPH 

       790        800        810        820        830        840 
LAEQCLAVLG GKIAGRETLV ARSAWPEFDP ALVVENEIVM PVQINGKKRG DLTIARDADQ 

       850        860        870 
ASIQQAVLEL DFVKAALNGS SPKKVIVVPQ RIVNVVA 

« Hide

References

[1]"Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts."
Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E.
J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000758 Genomic DNA. Translation: ABS13815.1.
RefSeqYP_001369644.1. NC_009667.1.

3D structure databases

ProteinModelPortalA6WXW1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING439375.Oant_1095.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS13815; ABS13815; Oant_1095.
GeneID5379321.
KEGGoan:Oant_1095.
PATRIC20467261. VBIOchAnt73124_1145.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycOANT439375:GJIT-1111-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_OCHA4
AccessionPrimary (citable) accession number: A6WXW1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries