A6WXV8 (ACSA_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	Oant_1092

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000065300
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	611	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6WXV8 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 6B8B09BA21A762B7	FASTA	651	72,591
	10 20 30 40 50 60 MSEKLYPVLA EAKRNTLIDN ATYLEWYQES VSDPDSFWAK HGRRIDWFKP FTKVKNTDFN 70 80 90 100 110 120 GDVSIKWYED GVTNVSYNCI DRHLKSRGDQ VAIIWEGDNP YIDKKITYRE LHENVCRLAN 130 140 150 160 170 180 VLKKHGVKKG DRVTIYLPMI PEAAYAMLAC ARIGAVHSVV FAGFSPEALA GRIVDCESTF 190 200 210 220 230 240 VITADEGVRG GKPVPLKENT DTAIDIAAKQ YVMVNKVLTV RRTGGKVSWG PGRDLWYHQE 250 260 270 280 290 300 VASVEPTCDP EPMNAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYASMTHQ YVFDYHDGDI 310 320 330 340 350 360 YWCTADVGWV TGHSYIVYGP LANGATTLMF EGVPNFPDQG RFWEVVDKHH VNIFYTAPTA 370 380 390 400 410 420 IRALMGAGDE FVTRSSRSTL RLLGSVGEPI NPEAWEWYYN VVGDQRSPIV DTWWQTETGG 430 440 450 460 470 480 ILITPLPGAT DLKPGSATRP FFGIKPELVD NEGAVIEGAV DGNLCIIDSW PGQMRTLYGD 490 500 510 520 530 540 HKRFIEAYFS TYKGKYFTGD GCRRDEDGYY WITGRVDDVL NISGHRLGTA EIESALVSHH 550 560 570 580 590 600 SVSEAAVVGY PHPIKGQGIY CYVTLMTGEA VQDEDALRKE LTQHVRKEIG PIATPDKIQF 610 620 630 640 650 SPGLPKTRSG KIMRRILRKI AEDEFGALGD TSTLADPGVV DDLIENRQNK K

References

[1]

"Complete sequence of chromosome 1 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000758 Genomic DNA. Translation: ABS13812.1.
RefSeq	YP_001369641.1. NC_009667.1.
3D structure databases
ProteinModelPortal	A6WXV8.
SMR	A6WXV8. Positions 17-648.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WXV8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5380140.
GenomeReviews	Gene locus Oant_1092 in contig CP000758_GR.
KEGG	oan:Oant_1092.
PATRIC	20467255. VBIOchAnt73124_1142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HBG547964.
OMA	KGKPYML.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	OANT439375:OANT_1092-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_OCHA4

Accession

Primary (citable) accession number: A6WXV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program