A6WXV2 (GLPK_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Oant_1086

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Glycerol kinase HAMAP MF_00186		PRO_1000020754
Regions
	Nucleotide binding	412 – 416	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	267	1	ATP By similarity
	Binding site	311	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6WXV2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 6F1026030451F63E	FASTA	499	54,897
	10 20 30 40 50 60 MTQFIGSIDQ GTTSSRFIIF DRQGDIVASD QREHEQIYPK AGWVEHNPIE IWRNTQHVIA 70 80 90 100 110 120 AALKKAKLKA SDIASVGITN QRETTLLWDR KTGAPLYNAI VWMDTRTDEL VSRYTKDGGA 130 140 150 160 170 180 DQLRAKTGLP ISTYFSGLKL RWILDNVPGA REKAEAGDAL FGTIDTWLVW NLTGGTEGGI 190 200 210 220 230 240 HITDVTNASR TQLMDLSTLQ WDEDILRLFD IPSACLPEIR SSSEVYGEIT LPSLSGVKLA 250 260 270 280 290 300 GILGDQQAAL FGQACLEPGE AKNTYGTGCF MLMNTGEKLV PSNYGLLTTV AYKLDGAKPV 310 320 330 340 350 360 YALEGSIAIT GALVQWLRDN LGIIRNSSDI ETLARTVEDN GDVYFVPAFS GLFAPHWQDS 370 380 390 400 410 420 ARGIIAGLTR FANKGHIARA ALEASAYQVR EVLDAMVKDS GVEITELRAD GGMTINELLM 430 440 450 460 470 480 QFQSDILDVP VVRPKIIETT ALGAAYAAGL AVGYWKSTKD IVENWQVGHR WHPRMSAEES 490 TRLFNAWEKA VQRSLGWIE

References

[1]

"Complete sequence of chromosome 1 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000758 Genomic DNA. Translation: ABS13806.1.
RefSeq	YP_001369635.1. NC_009667.1.
3D structure databases
ProteinModelPortal	A6WXV2.
SMR	A6WXV2. Positions 4-499.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WXV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5379515.
GenomeReviews	Gene locus Oant_1086 in contig CP000758_GR.
KEGG	oan:Oant_1086.
PATRIC	20467241. VBIOchAnt73124_1135.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	ATYFSGT.
ProtClustDB	CLSK978155.
Enzyme and pathway databases
BioCyc	OANT439375:OANT_1086-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_OCHA4

Accession

Primary (citable) accession number: A6WXV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program