A6WXS2 (PROA_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Oant_1056

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049973

Sequences

	Sequence		Length	Mass (Da)
	A6WXS2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 34231A56044D2A10	FASTA	427	44,615
	10 20 30 40 50 60 MLTKAETAND IAAVMAEVGR KARAAAAPLS IATTEQKNRA LIAAADAMLE ARGDILEANK 70 80 90 100 110 120 LDLANAEKNG MAASFVDRLA LDDGRISAIA DGIRAIAALP DPVGEVIAEW DRPNGLHIER 130 140 150 160 170 180 VRTPLGVIGV IYESRPNVTA DAGALCLKAG NAVILRGGSD SAHSSAAIHK ALVRGLEAAG 190 200 210 220 230 240 LPADAIQIVP VTDRAAVGEM LKGLDGAIDV IVPRGGKSLV ARVQSEARVP VFAHLEGICH 250 260 270 280 290 300 LYIDKSADLD MARKIAVDAK MRRTGICGAA ETLLVDRAAA AMHLVPILED LAAKSCEIRG 310 320 330 340 350 360 SADVLALYPA AKPATEEDWS TEYLDAIISV ALVDGISGAI EHINRYSSHH TEAVVAEDAA 370 380 390 400 410 420 AVARFFNEID SAILLHNAST QFADGGEFGM GAEIGIATGK MHARGPVGVE QLTSFKYRVR GDGQIRG

References

[1]

"Complete sequence of chromosome 1 of Ochrobactrum anthropi ATCC 49188." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000758 Genomic DNA. Translation: ABS13776.1.
RefSeq	YP_001369605.1. NC_009667.1.
3D structure databases
ProteinModelPortal	A6WXS2.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WXS2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5378532.
GenomeReviews	Gene locus Oant_1056 in contig CP000758_GR.
KEGG	oan:Oant_1056.
PATRIC	20467179. VBIOchAnt73124_1104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	OANT439375:OANT_1056-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_OCHA4

Accession

Primary (citable) accession number: A6WXS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program