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A6WWT4 (A6WWT4_OCHA4) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103
Short name=Fapy-DNA glycosylase HAMAP-Rule MF_00103
EC=3.2.2.23 HAMAP-Rule MF_00103
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM HAMAP-Rule MF_00103
Gene names
Name:mutM HAMAP-Rule MF_00103
Synonyms:fpg HAMAP-Rule MF_00103
Ordered Locus Names:Oant_0716 EMBL ABS13438.1
OrganismOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP] EMBL ABS13438.1
Taxonomic identifier439375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum ›

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103 SAAS SAAS015886

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103 SAAS SAAS015886

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103 SAAS SAAS015886

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103 SAAS SAAS015886

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00103 SAAS SAAS015886

Sequence similarities

Belongs to the FPG family. HAMAP-Rule MF_00103

Contains 1 FPG-type zinc finger. HAMAP-Rule MF_00103 SAAS SAAS015886

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Zinc finger266 – 30237FPG-type By similarity HAMAP-Rule MF_00103

Sites

Active site111Schiff-base intermediate with DNA By similarity HAMAP-Rule MF_00103
Active site121Proton donor By similarity HAMAP-Rule MF_00103
Active site671Proton donor; for beta-elimination activity By similarity HAMAP-Rule MF_00103
Active site2921Proton donor; for delta-elimination activity By similarity HAMAP-Rule MF_00103
Binding site1131DNA By similarity HAMAP-Rule MF_00103
Binding site1361DNA By similarity HAMAP-Rule MF_00103
Binding site1791DNA By similarity HAMAP-Rule MF_00103

Sequences

Sequence LengthMass (Da)Tools
A6WWT4 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 790EA251CB0B6253

FASTA30233,071
        10         20         30         40         50         60 
MALVQDQFSM PELPEVETVR RGLQPVMEGA TVSQVEQRRP DLRFPFPDHF VERLSGRRIS 

        70         80         90        100        110        120 
ALGRRAKYLT IHLDDGLSVI SHLGMSGSFR IETDDDGGTP GEFHHERSKL SAHDHVVFHL 

       130        140        150        160        170        180 
LRADGAPARI VYNDPRRFGF MLFAEEGTLD SHPLLASLGI EPTGNLLSGT ILAELFKGRR 

       190        200        210        220        230        240 
TPLKAALLDQ RLIAGLGNIY VCEALWRSHL SPMQTAGSVA QNPAIMEQLA ADIRTVIAEA 

       250        260        270        280        290        300 
IAAGGSTLKD YIQADGALGY FQHSFSVYGR EGEPCKNPAC NGIVERAVQS GRSTFFCASC 


QQ

« Hide

References

[1]"Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts."
Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E.
J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000758 Genomic DNA. Translation: ABS13438.1.
RefSeqYP_001369267.1. NC_009667.1.

3D structure databases

ProteinModelPortalA6WWT4.
ModBaseSearch...

Protein-protein interaction databases

STRING439375.Oant_0716.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS13438; ABS13438; Oant_0716.
GeneID5380282.
KEGGoan:Oant_0716.
PATRIC20466461. VBIOchAnt73124_0754.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0266.
HOGENOMHOG000020881.
KOK10563.
OMARREKFMN.
ProtClustDBPRK01103.

Enzyme and pathway databases

BioCycOANT439375:GJIT-723-MONOMER.

Family and domain databases

HAMAPMF_00103. Fapy_DNA_glycosyl.
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.
TIGRFAMsTIGR00577. fpg. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA6WWT4_OCHA4
AccessionPrimary (citable) accession number: A6WWT4
Entry history
Integrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info