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A6WWT4

- A6WWT4_OCHA4

UniProt

A6WWT4 - A6WWT4_OCHA4

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Protein

Formamidopyrimidine-DNA glycosylase

Gene
mutM, fpg, Oant_0716
Organism
Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.UniRule annotationSAAS annotations
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotations

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111Schiff-base intermediate with DNA By similarityUniRule annotation
Active sitei12 – 121Proton donor By similarityUniRule annotation
Active sitei67 – 671Proton donor; for beta-elimination activity By similarityUniRule annotation
Binding sitei113 – 1131DNA By similarityUniRule annotation
Binding sitei136 – 1361DNA By similarityUniRule annotation
Binding sitei179 – 1791DNA By similarityUniRule annotation
Active sitei292 – 2921Proton donor; for delta-elimination activity By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri266 – 30237FPG-type By similarityUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationSAAS annotationsImported, Hydrolase, LyaseUniRule annotationSAAS annotations

Keywords - Biological processi

DNA damage, DNA repairUniRule annotationSAAS annotations

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotations, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciOANT439375:GJIT-723-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formamidopyrimidine-DNA glycosylaseUniRule annotation (EC:3.2.2.23UniRule annotation)
Short name:
Fapy-DNA glycosylaseUniRule annotation
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM
Gene namesi
Name:mutMUniRule annotation
Synonyms:fpgUniRule annotation
Ordered Locus Names:Oant_0716Imported
OrganismiOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)Imported
Taxonomic identifieri439375 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum
ProteomesiUP000002301: Chromosome 1

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotationSAAS annotations

Protein-protein interaction databases

STRINGi439375.Oant_0716.

Structurei

3D structure databases

ProteinModelPortaliA6WWT4.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.UniRule annotation
Contains 1 FPG-type zinc finger.UniRule annotation
Contains FPG-type zinc finger.SAAS annotations

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri266 – 30237FPG-type By similarityUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-fingerUniRule annotationSAAS annotations

Phylogenomic databases

eggNOGiCOG0266.
HOGENOMiHOG000020881.
KOiK10563.
OMAiIYCSESL.
OrthoDBiEOG6QP131.

Family and domain databases

HAMAPiMF_00103. Fapy_DNA_glycosyl.
InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsiTIGR00577. fpg. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6WWT4-1 [UniParc]FASTAAdd to Basket

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MALVQDQFSM PELPEVETVR RGLQPVMEGA TVSQVEQRRP DLRFPFPDHF    50
VERLSGRRIS ALGRRAKYLT IHLDDGLSVI SHLGMSGSFR IETDDDGGTP 100
GEFHHERSKL SAHDHVVFHL LRADGAPARI VYNDPRRFGF MLFAEEGTLD 150
SHPLLASLGI EPTGNLLSGT ILAELFKGRR TPLKAALLDQ RLIAGLGNIY 200
VCEALWRSHL SPMQTAGSVA QNPAIMEQLA ADIRTVIAEA IAAGGSTLKD 250
YIQADGALGY FQHSFSVYGR EGEPCKNPAC NGIVERAVQS GRSTFFCASC 300
QQ 302
Length:302
Mass (Da):33,071
Last modified:August 21, 2007 - v1
Checksum:i790EA251CB0B6253
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000758 Genomic DNA. Translation: ABS13438.1.
RefSeqiWP_012090967.1. NC_009667.1.
YP_001369267.1. NC_009667.1.

Genome annotation databases

EnsemblBacteriaiABS13438; ABS13438; Oant_0716.
GeneIDi5380282.
KEGGioan:Oant_0716.
PATRICi20466461. VBIOchAnt73124_0754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000758 Genomic DNA. Translation: ABS13438.1 .
RefSeqi WP_012090967.1. NC_009667.1.
YP_001369267.1. NC_009667.1.

3D structure databases

ProteinModelPortali A6WWT4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 439375.Oant_0716.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS13438 ; ABS13438 ; Oant_0716 .
GeneIDi 5380282.
KEGGi oan:Oant_0716.
PATRICi 20466461. VBIOchAnt73124_0754.

Phylogenomic databases

eggNOGi COG0266.
HOGENOMi HOG000020881.
KOi K10563.
OMAi IYCSESL.
OrthoDBi EOG6QP131.

Enzyme and pathway databases

BioCyci OANT439375:GJIT-723-MONOMER.

Family and domain databases

HAMAPi MF_00103. Fapy_DNA_glycosyl.
InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsi TIGR00577. fpg. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts."
    Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E.
    J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Entry informationi

Entry nameiA6WWT4_OCHA4
AccessioniPrimary (citable) accession number: A6WWT4
Entry historyi
Integrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotations

External Data

Dasty 3

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