A6WWI2 (MTB1_OCHA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BabI
Short name=M.BabI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog

Gene names

Name:	ccrM
Ordered Locus Names:	Oant_0605

Organism

Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]

Taxonomic identifier

439375 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum › Ochrobactrum anthropi

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Modification methylase BabI		PRO_0000363194

Sequences

	Sequence		Length	Mass (Da)
	A6WWI2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 0D0B34E4710E708B	FASTA	377	42,079
	10 20 30 40 50 60 MSLVRLAHEL PIEAPRTAWL DSIIKGDCVA ALERLPDHSV DVIFADPPYN LQLGGDLHRP 70 80 90 100 110 120 DQSMVSAVDD HWDQFESFQA YDAFTRAWLM ACRRVLKPNG TIWVIGSYHN IFRVGSQLQD 130 140 150 160 170 180 LGFWLLNDVI WRKTNPMPNF RGRRFQNAHE TLIWASRDQK GKGYTFNYEA MKAANDDVQM 190 200 210 220 230 240 RSDWLFPICT GSERLKDENG DKVHPTQKPE ALLARIMMAS SKPGDVILDP FFGSGTTGAV 250 260 270 280 290 300 AKRLGRHFVG IEREQSYIDA ATARIDAVEP LGKAELTVMT GKRAEPRVAF TSVLEAGLLR 310 320 330 340 350 360 PGTVLCDERR RFAAIVRADG TLAANGEAGS IHRIGAKVQG FDACNGWTFW HYEENGALKP 370 IDALRKVIRD QMAVAGA

References

[1]

"Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts." Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E. J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000758 Genomic DNA. Translation: ABS13336.1.
RefSeq	YP_001369165.1. NC_009667.1.
3D structure databases
ProteinModelPortal	A6WWI2.
ModBase	Search...
Protein-protein interaction databases
STRING	439375.Oant_0605.
Protein family/group databases
REBASE	15992. M.OanORF605P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS13336; ABS13336; Oant_0605.
GeneID	5379433.
KEGG	oan:Oant_0605.
PATRIC	20466241. VBIOchAnt73124_0645.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0863.
HOGENOM	HOG000125329.
KO	K13581.
OMA	KVHSTQK.
ProtClustDB	CLSK2518600.
Enzyme and pathway databases
BioCyc	OANT439375:GJIT-611-MONOMER.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_OCHA4

Accession

Primary (citable) accession number: A6WWI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program