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Protein

4-hydroxyproline 2-epimerase 1

Gene

Oant_0439

Organism
Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of trans-4-hydroxy-L-proline (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp) in vitro, albeit with low efficiency. The physiological substrate may be different. Displays no proline racemase activity.Curated1 Publication

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

Kineticsi

kcat is 0.064 sec(-1) for t4LHyp epimerization.1 Publication

Manual assertion based on experiment ini

  1. KM=1.3 mM for trans-4-hydroxy-L-proline1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei90Proton acceptorBy similarity1
    Binding sitei251SubstrateBy similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxyproline 2-epimerase 11 Publication (EC:5.1.1.81 Publication)
    Short name:
    4Hyp 2-epimerase 1
    Short name:
    4HypE 11 Publication
    Gene namesi
    Ordered Locus Names:Oant_0439Imported
    OrganismiOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168)
    Taxonomic identifieri439375 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum
    Proteomesi
    • UP000002301 Componenti: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004322481 – 3434-hydroxyproline 2-epimerase 1Add BLAST343

    Interactioni

    Protein-protein interaction databases

    STRINGi439375.Oant_0439.

    Structurei

    Secondary structure

    1343
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi6 – 14Combined sources9
    Beta strandi20 – 25Combined sources6
    Helixi34 – 44Combined sources11
    Helixi46 – 52Combined sources7
    Beta strandi63 – 67Combined sources5
    Beta strandi75 – 81Combined sources7
    Helixi91 – 103Combined sources13
    Beta strandi111 – 120Combined sources10
    Beta strandi123 – 132Combined sources10
    Beta strandi135 – 142Combined sources8
    Beta strandi146 – 156Combined sources11
    Beta strandi162 – 179Combined sources18
    Turni180 – 182Combined sources3
    Helixi194 – 208Combined sources15
    Beta strandi214 – 216Combined sources3
    Beta strandi223 – 227Combined sources5
    Beta strandi231 – 233Combined sources3
    Beta strandi236 – 238Combined sources3
    Beta strandi241 – 244Combined sources4
    Helixi256 – 268Combined sources13
    Beta strandi277 – 279Combined sources3
    Beta strandi290 – 298Combined sources9
    Beta strandi301 – 310Combined sources10
    Beta strandi312 – 321Combined sources10

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4K8LX-ray1.90A1-343[»]
    ProteinModelPortaliA6WW16.
    SMRiA6WW16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni91 – 92Substrate bindingBy similarity2
    Regioni256 – 257Substrate bindingBy similarity2

    Sequence similaritiesi

    Belongs to the proline racemase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CTU. Bacteria.
    COG3938. LUCA.
    HOGENOMiHOG000084336.
    OMAiWITGIHQ.
    OrthoDBiPOG091H0FIS.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6WW16-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRSTKVIHIV GCHAEGEVGD VIVGGVAPPP GKTVWEQSRF IASDETLRNF
    60 70 80 90 100
    VLNEPRGGVF RHVNLLVPPK DPRAQMGFII MEPADTPPMS GSNSICVSTV
    110 120 130 140 150
    LLDSGIIPMQ EPVTRMVLEA PGGLIEVEAE CRNGKAERIS VRNVPSFADR
    160 170 180 190 200
    LNASLEVEGL GTITVDTAYG GDSFVIVDAA SIGMKIEPGQ ARELAEIGVK
    210 220 230 240 250
    ITKAANEQLG FRHPEKDWNH ISFCQITEPV TRDGDILTGV NTVAIRPAKL
    260 270 280 290 300
    DRSPTGTGCS ARMAVLHAKG QMKVGERFIG KSVLGTEFHC RLDKTLELGG
    310 320 330 340
    KPAISPIISG RAWVTGTSQL MLDPSDPFPS GYRLSDTWPN MPE
    Length:343
    Mass (Da):36,899
    Last modified:August 21, 2007 - v1
    Checksum:iADE36139FF040D81
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000758 Genomic DNA. Translation: ABS13170.1.
    RefSeqiWP_012090731.1. NC_009667.1.

    Genome annotation databases

    EnsemblBacteriaiABS13170; ABS13170; Oant_0439.
    GeneIDi5380754.
    KEGGioan:Oant_0439.
    PATRICi20465887. VBIOchAnt73124_0468.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000758 Genomic DNA. Translation: ABS13170.1.
    RefSeqiWP_012090731.1. NC_009667.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4K8LX-ray1.90A1-343[»]
    ProteinModelPortaliA6WW16.
    SMRiA6WW16.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi439375.Oant_0439.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABS13170; ABS13170; Oant_0439.
    GeneIDi5380754.
    KEGGioan:Oant_0439.
    PATRICi20465887. VBIOchAnt73124_0468.

    Phylogenomic databases

    eggNOGiENOG4105CTU. Bacteria.
    COG3938. LUCA.
    HOGENOMiHOG000084336.
    OMAiWITGIHQ.
    OrthoDBiPOG091H0FIS.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry namei4HPE1_OCHA4
    AccessioniPrimary (citable) accession number: A6WW16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 4, 2015
    Last sequence update: August 21, 2007
    Last modified: November 2, 2016
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.