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Protein

4-hydroxyproline 2-epimerase 1

Gene

Oant_0439

Organism
Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of trans-4-hydroxy-L-proline (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp) in vitro, albeit with low efficiency. The physiological substrate may be different. Displays no proline racemase activity.Curated1 Publication

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

Kineticsi

kcat is 0.064 sec(-1) for t4LHyp epimerization.1 Publication

  1. KM=1.3 mM for trans-4-hydroxy-L-proline1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901Proton acceptorBy similarity
    Binding sitei251 – 2511SubstrateBy similarity

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciOANT439375:GJIT-445-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxyproline 2-epimerase 11 Publication (EC:5.1.1.81 Publication)
    Short name:
    4Hyp 2-epimerase 1
    Short name:
    4HypE 11 Publication
    Gene namesi
    Ordered Locus Names:Oant_0439Imported
    OrganismiOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
    Taxonomic identifieri439375 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum
    Proteomesi
    • UP000002301 Componenti: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3433434-hydroxyproline 2-epimerase 1PRO_0000432248Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi439375.Oant_0439.

    Structurei

    Secondary structure

    1
    343
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 149Combined sources
    Beta strandi20 – 256Combined sources
    Helixi34 – 4411Combined sources
    Helixi46 – 527Combined sources
    Beta strandi63 – 675Combined sources
    Beta strandi75 – 817Combined sources
    Helixi91 – 10313Combined sources
    Beta strandi111 – 12010Combined sources
    Beta strandi123 – 13210Combined sources
    Beta strandi135 – 1428Combined sources
    Beta strandi146 – 15611Combined sources
    Beta strandi162 – 17918Combined sources
    Turni180 – 1823Combined sources
    Helixi194 – 20815Combined sources
    Beta strandi214 – 2163Combined sources
    Beta strandi223 – 2275Combined sources
    Beta strandi231 – 2333Combined sources
    Beta strandi236 – 2383Combined sources
    Beta strandi241 – 2444Combined sources
    Helixi256 – 26813Combined sources
    Beta strandi277 – 2793Combined sources
    Beta strandi290 – 2989Combined sources
    Beta strandi301 – 31010Combined sources
    Beta strandi312 – 32110Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4K8LX-ray1.90A1-343[»]
    ProteinModelPortaliA6WW16.
    SMRiA6WW16. Positions 1-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni91 – 922Substrate bindingBy similarity
    Regioni256 – 2572Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the proline racemase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CTU. Bacteria.
    COG3938. LUCA.
    HOGENOMiHOG000084336.
    OMAiWITGIHQ.
    OrthoDBiEOG6SNDV3.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6WW16-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRSTKVIHIV GCHAEGEVGD VIVGGVAPPP GKTVWEQSRF IASDETLRNF
    60 70 80 90 100
    VLNEPRGGVF RHVNLLVPPK DPRAQMGFII MEPADTPPMS GSNSICVSTV
    110 120 130 140 150
    LLDSGIIPMQ EPVTRMVLEA PGGLIEVEAE CRNGKAERIS VRNVPSFADR
    160 170 180 190 200
    LNASLEVEGL GTITVDTAYG GDSFVIVDAA SIGMKIEPGQ ARELAEIGVK
    210 220 230 240 250
    ITKAANEQLG FRHPEKDWNH ISFCQITEPV TRDGDILTGV NTVAIRPAKL
    260 270 280 290 300
    DRSPTGTGCS ARMAVLHAKG QMKVGERFIG KSVLGTEFHC RLDKTLELGG
    310 320 330 340
    KPAISPIISG RAWVTGTSQL MLDPSDPFPS GYRLSDTWPN MPE
    Length:343
    Mass (Da):36,899
    Last modified:August 21, 2007 - v1
    Checksum:iADE36139FF040D81
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000758 Genomic DNA. Translation: ABS13170.1.
    RefSeqiWP_012090731.1. NC_009667.1.

    Genome annotation databases

    EnsemblBacteriaiABS13170; ABS13170; Oant_0439.
    GeneIDi5380754.
    KEGGioan:Oant_0439.
    PATRICi20465887. VBIOchAnt73124_0468.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000758 Genomic DNA. Translation: ABS13170.1.
    RefSeqiWP_012090731.1. NC_009667.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4K8LX-ray1.90A1-343[»]
    ProteinModelPortaliA6WW16.
    SMRiA6WW16. Positions 1-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi439375.Oant_0439.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABS13170; ABS13170; Oant_0439.
    GeneIDi5380754.
    KEGGioan:Oant_0439.
    PATRICi20465887. VBIOchAnt73124_0468.

    Phylogenomic databases

    eggNOGiENOG4105CTU. Bacteria.
    COG3938. LUCA.
    HOGENOMiHOG000084336.
    OMAiWITGIHQ.
    OrthoDBiEOG6SNDV3.

    Enzyme and pathway databases

    BioCyciOANT439375:GJIT-445-MONOMER.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts."
      Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E.
      J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49188 / DSM 6882 / NCTC 12168.
    2. "Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks."
      Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B., San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A., Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.
      Elife 3:0-0(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry namei4HPE1_OCHA4
    AccessioniPrimary (citable) accession number: A6WW16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 4, 2015
    Last sequence update: August 21, 2007
    Last modified: May 11, 2016
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.