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A6WV84

- GLND_OCHA4

UniProt

A6WV84 - GLND_OCHA4

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, Oant_0157
Organism
Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciOANT439375:GJIT-159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Oant_0157
OrganismiOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
Taxonomic identifieri439375 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum
ProteomesiUP000002301: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000022349Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi439375.Oant_0157.

Structurei

3D structure databases

ProteinModelPortaliA6WV84.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini496 – 613118HDAdd
BLAST
Domaini737 – 81983ACT 1Add
BLAST
Domaini848 – 93184ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 379379UridylyltransferaseUniRule annotationAdd
BLAST
Regioni380 – 736357Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6WV84-1 [UniParc]FASTAAdd to Basket

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MSAHDLKLEE IVNPETLRRK LNELADSTDE NYTSPTVRKV VLQALKDALV    50
RGRANAEGML MKDGGGTLCA KRLSFLMDTL IEALFEFATT KAYPMINPSK 100
AENMAIVAVG GYGRGGLAPG SDIDLLFLLP YKQTPWGEQV VEYMLYMLWD 150
MGLKVGHSTR NIDECIRLSR EDMTIRTAIL DARFLTGNRE LFKTLVTRFD 200
EEIVKDTGPE FIQAKLAERD QRHRKAGETR YLVEPNVKEG KGGQRDLHTL 250
FWITKYFYRV KTKEELVKLG VLSRAELKLF NKAEDFLWAV RCHMHFATLK 300
AEERLSFDIQ PEIAQRLGYT AHPGQNYVER FMKHYFLVAK DVGDLTRIIS 350
AALEEQQAKH VPGFNRIFLT FSRRKRKLSA DGDFVSENHR INIARPEVFK 400
EDPVNIIRLF HLADKHGLEF HPEAMQSLTR SLKLINSDLR ENPEANKLFL 450
EILTSPRNPE LILRRMNESG VLGKFIPDFG KIVAMMQFNM YHHYTVDEHL 500
LRCIAVLSEI EHGELENEHP LSNHLITTVK RDRNLLYVAL LLHDIAKGRP 550
EDHSVAGARI ARRLGPRLGL TPTETETVEW LVREHLTMSM VAQSRDLNDR 600
KTIIDFADTV QTMERLKLLL ILTVCDIKAV GPGVWNGWKG QLLRTLFYET 650
ELVLTGGFSE LSRADRDHQA REALADRLSD WPKDKRDAYL ALHYTNYFLT 700
VSLEDQVRHA HFIREADRNE RALATMAKPH TFEAVTEITV LAPDHPRLLS 750
IITGACAAAG ANIVDAQIFT TGDGRALDTI LISREFDTDD DERRRAERVG 800
KVIEDVLSGK AHLPDVLAKR TKPKRAAKAF KVEPRVEINN TLSNKFTVIE 850
VEGLDRPGLL SELTGLISDL SLDIASAHIT TFGEKVIDSF YVTDLVGHKI 900
SNATRQGNIR RKLLGVLSGE NGSKTNGRSS QAAA 934
Length:934
Mass (Da):105,778
Last modified:August 21, 2007 - v1
Checksum:iE614E52BF69FF8E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000758 Genomic DNA. Translation: ABS12888.1.
RefSeqiWP_011982362.1. NC_009667.1.
YP_001368717.1. NC_009667.1.

Genome annotation databases

EnsemblBacteriaiABS12888; ABS12888; Oant_0157.
GeneIDi5379798.
KEGGioan:Oant_0157.
PATRICi20465277. VBIOchAnt73124_0167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000758 Genomic DNA. Translation: ABS12888.1 .
RefSeqi WP_011982362.1. NC_009667.1.
YP_001368717.1. NC_009667.1.

3D structure databases

ProteinModelPortali A6WV84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 439375.Oant_0157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS12888 ; ABS12888 ; Oant_0157 .
GeneIDi 5379798.
KEGGi oan:Oant_0157.
PATRICi 20465277. VBIOchAnt73124_0167.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci OANT439375:GJIT-159-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts."
    Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M., Ugalde R.A., Garcia E., Tolmasky M.E.
    J. Bacteriol. 193:4274-4275(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49188 / DSM 6882 / NCTC 12168.

Entry informationi

Entry nameiGLND_OCHA4
AccessioniPrimary (citable) accession number: A6WV84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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