ID   FTHS_SHEB8              Reviewed;         585 AA.
AC   A6WSY9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   27-MAR-2024, entry version 76.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Shew185_3804;
OS   Shewanella baltica (strain OS185).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=402882;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OS185;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Brettar I., Rodrigues J., Konstantinidis K., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome of Shewanella baltica OS185.";
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000753; ABS09928.1; -; Genomic_DNA.
DR   RefSeq; WP_012090278.1; NC_009665.1.
DR   AlphaFoldDB; A6WSY9; -.
DR   SMR; A6WSY9; -.
DR   KEGG; sbm:Shew185_3804; -.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..585
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000068794"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   585 AA;  61477 MW;  63780632AA87C3E9 CRC64;
     MLTDMNISSR ASLKNITELG ADLGLLPEEM MLFGHTKAKV ELSVLQRLAG QRKGKLIIVT
     AVTPTPHGEG KTVTSIGLTQ SLNAIGQKAC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
     EMNLHLTGDI HAVSSAHNLG AAAIAARLFH EARLGKKEFE AQSGQAFLAI DPNEIRWHRV
     VDHNDRCLRQ IHVGLGDNNG PEYESSFDIT AASELMAILA LSHDLADMRA RIGRLVLALN
     TQGQVITADD LGVAGAMTAI MADAIKPTLM QTLNGSPCLI HSGPFANIAH GNSSIIADDI
     ALRLADFVVT EGGFGSDMGF EKFCNIKVRQ SGQAPAAAVL VTTLKALKAN SGLATEVDSN
     VSNIHVPNIN ATNINAPDQA RLEAGFANLN WHINNVARYG IPVVVAINRF ATDSDAELQW
     LMEAVNASAA FGCEISDAFS QGEAGAIALA QTVVRAAETE SQFKLLYPDE VSLEAKLSTL
     AEVGYGAAGV SLSIEAKQQA QQLTALGYGH LPLCMAKTPL SISHDPSLKG VPKDFVVPVR
     ELVLHAGAGF ITALVGNVMT MPGLGLKPGY LKIDIDAKGE IVGLG
//