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A6WSH0 (MAO1_SHEB8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NAD-dependent malic enzyme
Short name=NAD-ME
EC=1.1.1.38
Gene names
Name:maeA
Ordered Locus Names:Shew185_3634
OrganismShewanella baltica (strain OS185) [Complete proteome] [HAMAP]
Taxonomic identifier402882 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Subunit structure

Homotetramer By similarity. HAMAP MF_01619

Sequence similarities

Belongs to the malic enzymes family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 562562NAD-dependent malic enzyme HAMAP MF_01619
PRO_1000069540

Sites

Active site1011Proton donor By similarity
Active site1721Proton acceptor By similarity
Metal binding2431Divalent metal cation By similarity
Metal binding2441Divalent metal cation By similarity
Metal binding2671Divalent metal cation By similarity
Binding site1541NAD By similarity
Binding site2671NAD By similarity
Binding site4151NAD By similarity
Site2671Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A6WSH0 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: E3523020D79A2CF8

FASTA56262,116
        10         20         30         40         50         60 
MDDNKRPLYL PFAGPAILEA PLINKGSAFS EEERIFFNLE GLVPYAIETI EEQASRAYDQ 

        70         80         90        100        110        120 
FRSFNNDLDK HIYLRNIQDT NETLFYRLVQ NNISEMMPII YTPTVGLACE RFSKNYRRNR 

       130        140        150        160        170        180 
GLFISYPNKD RIDDILNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG 

       190        200        210        220        230        240 
GISPAYTLPI TLDVGTDNPQ LLEDPMYMGW RHPRIGGEEY AEFIEAFMQA VHVRWPDTLI 

       250        260        270        280        290        300 
QFEDFAQKNA MPILERYKDR YCCFNDDIQG TAAVAVGSLL AACKAAGTEL NQQRIAFLGA 

       310        320        330        340        350        360 
GSAGCGIAEA IVAQMVSEGI SDEQARTQVC MVDRWGLLLD NMPNLLPFQQ KLAQKCTNIQ 

       370        380        390        400        410        420 
NWSNFSDNIS LLDVVNNTKP TVLIGVSGVP GLFTEEIIRA MHSHCARPII FPLSNPTSRV 

       430        440        450        460        470        480 
EATPKDILHW TSGQALVATG SPFEPVVVDG ETFEIAQCNN SFIFPGIGLG VLASGARHVS 

       490        500        510        520        530        540 
DAMLMASSRA LAECSPLAID GSGPLLPKLE DIHAVSKHIA FAVGKVAVEQ GLTLPMSDEI 

       550        560 
LQQSIEGNFW SPEYRRYKRT SF

« Hide

References

[1]"Complete sequence of chromosome of Shewanella baltica OS185."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Brettar I., Rodrigues J., Konstantinidis K., Tiedje J., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS185.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000753 Genomic DNA. Translation: ABS09759.1.
RefSeqYP_001367822.1. NC_009665.1.

3D structure databases

ProteinModelPortalA6WSH0.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6WSH0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5369452.
GenomeReviewsGene locus Shew185_3634 in contig CP000753_GR.
KEGGsbm:Shew185_3634.
PATRIC23464329. VBISheBal127872_3911.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0281.
HOGENOMHBG289821.
OMAFYRLMQD.
ProtClustDBPRK13529.

Enzyme and pathway databases

BioCycSBAL402882:SHEW185_3634-MONOMER.

Family and domain databases

HAMAPMF_01619. NAD_malic_enz.
[Tree]
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR023667. NAD_malic_enz_proteobac.
[Graphical view]
Gene3DG3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00027.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAO1_SHEB8
AccessionPrimary (citable) accession number: A6WSH0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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