A6WQ07 (PDXB_SHEB8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Erythronate-4-phosphate dehydrogenase
EC=1.1.1.290

Gene names

Name:	pdxB
Ordered Locus Names:	Shew185_2762

Organism

Shewanella baltica (strain OS185) [Complete proteome] [HAMAP]

Taxonomic identifier

402882 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella baltica

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate By similarity. HAMAP-Rule MF_01825
Catalytic activity	4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH. HAMAP-Rule MF_01825
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5. HAMAP-Rule MF_01825
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01825
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01825.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-phosphoerythronate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Erythronate-4-phosphate dehydrogenase HAMAP-Rule MF_01825		PRO_1000070407
Sites
	Active site	209	1	By similarity
	Active site	238	1	By similarity
	Active site	255	1	Proton donor By similarity
	Binding site	45	1	Substrate By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	147	1	NAD By similarity
	Binding site	233	1	NAD By similarity
	Binding site	258	1	NAD; via amide nitrogen By similarity
	Binding site	259	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6WQ07 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 464CCA52ACD27D03	FASTA	376	41,362
	10 20 30 40 50 60 MKILVDENMP YVEPLFGDLG DIIPVNGRTL TAEQVRDADV LLVRSVTKVN AELLSGNNKL 70 80 90 100 110 120 KFVGSATIGT DHVDLAYLAE RNIPFSNAPG CNATAVGEFA FIAMLELAQR FNSPLKGKVV 130 140 150 160 170 180 GIVGAGNTGT ATAKCLQAYG IKVLLNDPIK AAEGDPRSFV SLDTITAQAD IISLHVPITR 190 200 210 220 230 240 TGEHKTKHLF DEARLKALKP NTWLVNCCRG DVIDNQALIK VKRQRDDLKL VLDVWEGEPT 250 260 270 280 290 300 PLPELVPLAE FATPHIAGYS LEGKARGTFM LYQKLCQLLN ITADKSLLDL LPTFNIKAVE 310 320 330 340 350 360 LATAPNEKAL LQLARFVYDL RDDDKMFRNT FLNENGFDTM RKNHQHRREF SALALAYDGQ 370 SEVDWLSNLG FSGVGQ

References

[1]

"Complete sequence of chromosome of Shewanella baltica OS185." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Brettar I., Rodrigues J., Konstantinidis K., Tiedje J., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS185.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000753 Genomic DNA. Translation: ABS08896.1.
RefSeq	YP_001366959.1. NC_009665.1.
3D structure databases
ProteinModelPortal	A6WQ07.
ModBase	Search...
Protein-protein interaction databases
STRING	402882.Shew185_2762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS08896; ABS08896; Shew185_2762.
GeneID	5371620.
KEGG	sbm:Shew185_2762.
PATRIC	23462425. VBISheBal127872_2998.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0111.
HOGENOM	HOG000234432.
KO	K03473.
OMA	SSAPGCN.
ProtClustDB	CLSK906984.
Enzyme and pathway databases
BioCyc	SBAL402882:GJ99-2843-MONOMER.
UniPathway	UPA00244; UER00310.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
HAMAP	MF_01825. PdxB.
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR020921. Erythronate-4-P_DHase. IPR024531. Erythronate-4-P_DHase_dimer. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10996:SF4. PTHR10996:SF4. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF11890. DUF3410. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXB_SHEB8

Accession

Primary (citable) accession number: A6WQ07

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program